Table 1. Calculated K m values for... | Rockefeller University Press

Table 1.

Calculated K_m values for kinases tested in the in vitro activity assay with ion titrations

	K_m [Mg²⁺] mM	K_m [Mn²⁺] mM	K_m [Ca²⁺] mM
ITK	0.71 ± 0.16	0.074 ± 0.04	0.036 ± 0.13
BTK	2.61 ± 0.51	0.333 ± 0.13	0.032 ± 0.1
Lck	2.80 ± 0.26
ZAP70	2.032 ± 0.54
ERK1/2	0.374 ± 0.2
DYRK	0.184 ± 0.05
PKCθ	0.260 ± 0.07
PKD2	0.265 ± 0.06

	K_m [Mg²⁺] mM	K_m [Mn²⁺] mM	K_m [Ca²⁺] mM
ITK	0.71 ± 0.16	0.074 ± 0.04	0.036 ± 0.13
BTK	2.61 ± 0.51	0.333 ± 0.13	0.032 ± 0.1
Lck	2.80 ± 0.26
ZAP70	2.032 ± 0.54
ERK1/2	0.374 ± 0.2
DYRK	0.184 ± 0.05
PKCθ	0.260 ± 0.07
PKD2	0.265 ± 0.06

Calculated K_m values for kinase assays with Mg²⁺, Mn²⁺, and Ca²⁺ for ITK and BTK. Calculated K_m values for kinase assays with Mg²⁺ for LCK, ZAP70, ERK, DYRK, PKCθ, and PKD2.

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