Table 2.
Primer pairs used for generating the IQGAP1 truncation constructs
Construct nameForward primerReverse primer
FL IQGAP1 nt1 SalI Kozak forward IQGAP1 nt4791 XmaI reverse no stop 
Δ5,6 IQGAP1 nt1 SalI Kozak forward IQGAP1 nt2850 XmaI reverse no stop 
Δ4-6 IQGAP1 nt1 SalI Kozak forward IQGAP1 nt2190 XmaI reverse no stop 
Δ1 IQGAP1 nt493 SalI Kozak forward IQGAP1 nt4791 XmaI reverse no stop 
Δ1,5,6 IQGAP1 nt493 SalI Kozak forward IQGAP1 nt2850 XmaI reverse no stop 
Δ1-3 IQGAP1 nt2188 Sal1 Kozak forward IQGAP1 nt4791 XmaI reverse no stop 
Δ4 IQGAP1 nt2590 iqmot4 del forward IQGAP1 nt2253 iqmot1 del reverse 
Construct nameForward primerReverse primer
FL IQGAP1 nt1 SalI Kozak forward IQGAP1 nt4791 XmaI reverse no stop 
Δ5,6 IQGAP1 nt1 SalI Kozak forward IQGAP1 nt2850 XmaI reverse no stop 
Δ4-6 IQGAP1 nt1 SalI Kozak forward IQGAP1 nt2190 XmaI reverse no stop 
Δ1 IQGAP1 nt493 SalI Kozak forward IQGAP1 nt4791 XmaI reverse no stop 
Δ1,5,6 IQGAP1 nt493 SalI Kozak forward IQGAP1 nt2850 XmaI reverse no stop 
Δ1-3 IQGAP1 nt2188 Sal1 Kozak forward IQGAP1 nt4791 XmaI reverse no stop 
Δ4 IQGAP1 nt2590 iqmot4 del forward IQGAP1 nt2253 iqmot1 del reverse 
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