Table 1.
Proteins involved in signaling and oxidant-related processes identified by MS in the material eluted from the anti-PCNA coimmunoprecipitation in neutrophil cytosol
Swiss-Prot IDProtein nameMolecular weightGene nameUniProt ID
Ubiquitin 
UBA1_HUMAN Ubiquitin-like modifier-activating enzyme 1 117.8 UBA1 P22314 
Cytoskeleton 
PROF1_HUMAN Profilin-1 15.1 PFN1 P07737 
FLNA_HUMAN Filamin-A 280.7 FLNA P21333 
MOES_HUMAN Moesin 67.8 MSN P26038 
VINC_HUMAN Vinculin 123.8 VCL P18206 
ARC1B_HUMAN Actin-related protein 2/3 complex subunit 1B 40.9 ARPC1B O15143 
Oxidant-related proteins 
PDIA1_HUMAN Protein disulfide-isomerase 57.1 P4HB P07237 
THIO_HUMAN Thioredoxin 11.7 TXN P10599 
GTP-binding protein 
IQGA1_HUMAN Ras GTPase-activating-like protein IQGAP1 189.3 IQGAP1 P46940 
GDIB_HUMAN Rab GDP dissociation inhibitor β 50.7 GDI2 P50395 
GDIR1_HUMAN Rho GDP-dissociation inhibitor 1 23.2 ARHGDIA P52565 
GDIR2_HUMAN Rho GDP-dissociation inhibitor 2 23 ARHGDIB P52566 
NADPH oxidase 
NCF1_HUMAN Neutrophil cytosol factor 1; p47phox 44.8 NCF1 P14598 
NCF2_HUMAN Neutrophil cytosol factor 2; p67phox 59.8 NCF2 P19878 
NCF4_HUMAN Neutrophil cytosol factor 4; p40phox 39 NCF4 Q15080 
COR1A_HUMAN Coronin-1A 51 CORO1A P31146 
G-coupled protein 
GNAI2_HUMAN Guanine nucleotide-binding protein G(i) subunit α-2 40.5 GNAI2 P04899 
Glycolysis 
G3P_HUMAN Glyceraldehyde-3-phosphate dehydrogenase 36.1 GAPDH P04406 
G6PD_HUMAN Glucose-6-phosphate 1-dehydrogenase 59.3 G6PD P11413 
G6PI_HUMAN Glucose-6-phosphate isomerase 63.1 GPI P06744 
6PGD_HUMAN 6-Phosphogluconate dehydrogenase 53.1 PGD P52209 
6PGL_HUMAN 6-Phosphogluconolactonase 27.5 PGLS O95336 
ALDOA_HUMAN Fructose-bisphosphate aldolase A 39.4 ALDOA P04075 
ALDOC_HUMAN Fructose-bisphosphate aldolase C 39.5 ALDOC P09972 
HXK1_HUMAN Hexokinase-1 102.5 HK1 P19367 
LDH6B_HUMAN l-lactate dehydrogenase A-like 6B 41.9 LDHAL6B Q9BYZ2 
LDHA_HUMAN l-lactate dehydrogenase A chain 36.7 LDHA P00338 
LDHB_HUMAN l-lactate dehydrogenase B chain 36.6 LDHB P07195 
KPYM_HUMAN Pyruvate kinase isozymes M1/M2 57.9 PKM P14618 
Swiss-Prot IDProtein nameMolecular weightGene nameUniProt ID
Ubiquitin 
UBA1_HUMAN Ubiquitin-like modifier-activating enzyme 1 117.8 UBA1 P22314 
Cytoskeleton 
PROF1_HUMAN Profilin-1 15.1 PFN1 P07737 
FLNA_HUMAN Filamin-A 280.7 FLNA P21333 
MOES_HUMAN Moesin 67.8 MSN P26038 
VINC_HUMAN Vinculin 123.8 VCL P18206 
ARC1B_HUMAN Actin-related protein 2/3 complex subunit 1B 40.9 ARPC1B O15143 
Oxidant-related proteins 
PDIA1_HUMAN Protein disulfide-isomerase 57.1 P4HB P07237 
THIO_HUMAN Thioredoxin 11.7 TXN P10599 
GTP-binding protein 
IQGA1_HUMAN Ras GTPase-activating-like protein IQGAP1 189.3 IQGAP1 P46940 
GDIB_HUMAN Rab GDP dissociation inhibitor β 50.7 GDI2 P50395 
GDIR1_HUMAN Rho GDP-dissociation inhibitor 1 23.2 ARHGDIA P52565 
GDIR2_HUMAN Rho GDP-dissociation inhibitor 2 23 ARHGDIB P52566 
NADPH oxidase 
NCF1_HUMAN Neutrophil cytosol factor 1; p47phox 44.8 NCF1 P14598 
NCF2_HUMAN Neutrophil cytosol factor 2; p67phox 59.8 NCF2 P19878 
NCF4_HUMAN Neutrophil cytosol factor 4; p40phox 39 NCF4 Q15080 
COR1A_HUMAN Coronin-1A 51 CORO1A P31146 
G-coupled protein 
GNAI2_HUMAN Guanine nucleotide-binding protein G(i) subunit α-2 40.5 GNAI2 P04899 
Glycolysis 
G3P_HUMAN Glyceraldehyde-3-phosphate dehydrogenase 36.1 GAPDH P04406 
G6PD_HUMAN Glucose-6-phosphate 1-dehydrogenase 59.3 G6PD P11413 
G6PI_HUMAN Glucose-6-phosphate isomerase 63.1 GPI P06744 
6PGD_HUMAN 6-Phosphogluconate dehydrogenase 53.1 PGD P52209 
6PGL_HUMAN 6-Phosphogluconolactonase 27.5 PGLS O95336 
ALDOA_HUMAN Fructose-bisphosphate aldolase A 39.4 ALDOA P04075 
ALDOC_HUMAN Fructose-bisphosphate aldolase C 39.5 ALDOC P09972 
HXK1_HUMAN Hexokinase-1 102.5 HK1 P19367 
LDH6B_HUMAN l-lactate dehydrogenase A-like 6B 41.9 LDHAL6B Q9BYZ2 
LDHA_HUMAN l-lactate dehydrogenase A chain 36.7 LDHA P00338 
LDHB_HUMAN l-lactate dehydrogenase B chain 36.6 LDHB P07195 
KPYM_HUMAN Pyruvate kinase isozymes M1/M2 57.9 PKM P14618 

Immunoprecipitation of PCNA was performed in cytosols from human neutrophils obtained by nitrogen cavitation, and proteins associated with PCNA were identified by MS. This representative experiment was performed two times on two different cytosols with similar results.

or Create an Account

Close Modal
Close Modal