Table 1.
List of antibodies used in IHC analysis of paraffin sections with related protocols
AntibodyManufacturerAutostainerDilutionAntigen retrievalBlock (min)Antibody incubationDetection kit used
USP28 (HPA006778) Sigma BOND-MAX 1/200 pH 9, 20 min 10 min 15 min Bond Polymer Refine Detection kit 
BRAF (F3) (SC-55522) Santa Cruz Benchmark XT Ready-to-use pH 9, 64 min None 16 min Optiview DAB IHC Detection kit 
AntibodyManufacturerAutostainerDilutionAntigen retrievalBlock (min)Antibody incubationDetection kit used
USP28 (HPA006778) Sigma BOND-MAX 1/200 pH 9, 20 min 10 min 15 min Bond Polymer Refine Detection kit 
BRAF (F3) (SC-55522) Santa Cruz Benchmark XT Ready-to-use pH 9, 64 min None 16 min Optiview DAB IHC Detection kit 
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