Table 1. Analysis of cell subsets in... | Rockefeller University Press

Table 1.

Analysis of cell subsets in response to T reg elimination and PRC2 inhibition

Parameter	FoxP3DTR	FoxP3DTR	Fold change	FoxP3DTR	Fold change	FoxP3DTR	Fold change
		+DTx		+DTx		+DTx
		+DTx		+GSK503		+Rescue
Lymph nodes
Total cell no.	1.18E+08	8.28E+08	7.01	3.40E+08	0.41	3.54E+08	0.43
±SEM	6.51E+06	7.53E+07	***	4.62E+07	***	4.80E+07	**
No. CD4⁺TCRb⁺	3.90E+07	2.50E+08	6.40	1.04E+08	0.42	9.90E+07	0.4
±SEM	3.11E+06	2.53E+07	***	1.85E+07	***	1.20E+07	*
No. CD8⁺TCRb⁺	3.72E+07	2.10E+08	5.66	8.94E+07	0.43	8.11E+07	0.39
±SEM	2.49E+06	1.80E+07	***	1.03E+07	***	1.25E+07	**
No. CD19⁺B220⁺	2.66E+07	1.72E+08	6.48	4.97E+07	0.29	1.40E+08	0.81
±SEM	5.30E+06	3.92E+07	**	1.23E+07	*	2.29E+07	NS
No. GR1^hiCD11b⁺	5.92E+05	1.77E+07	29.9	3.84E+06	0.22	7.65E+06	0.43
±SEM	2.14E+05	3.29E+06	***	1.04E+06	**	1.75E+06	NS
No. F4/80⁺ CD11c⁻	1.10E+06	1.11E+07	10.11	2.53E+06	0.23	6.32E+06	0.57
±SEM	3.91E+05	1.90E+06	***	9.77E+05	**	2.75E+06	NS
Spleen
Total cell no.	1.22E+08	6.41E+08	5.26	2.04E+08	0.32	2.36E+08	0.37
±SEM	1.34E+07	5.82E+07	***	3.48E+07	***	9.25E+07	**
No. CD4⁺TCRb⁺	1.90E+07	1.40E+08	7.36	3.62E+07	0.26	7.00E+07	0.5
±SEM	2.67E+06	1.45E+07	***	8.52E+06	***	2.62E+07	*
No. CD8⁺TCRb⁺	1.86E+07	1.13E+08	6.09	2.71E+07	0.24	4.94E+07	0.44
±SEM	1.61E+06	1.45E+07	***	6.61E+06	***	2.23E+07	NS
No. CD19⁺B220⁺	4.93E+07	1.09E+08	2.22	3.58E+07	0.33	6.94E+07	0.64
±SEM	9.86E+06	1.65E+07	*	1.01E+07	**	2.48E+07	NS
No. GR1^hiCD11b⁺	2.99E+06	5.95E+07	20.56	3.44E+07	0.58	2.48E+07	0.42
±SEM	1.21E+06	1.19E+07	***	5.72E+06	NS	1.14E+07	NS
No. F4/80⁺CD11c−	2.06E+06	5.57E+07	27.12	3.13E+07	0.56	2.19E+07	0.39
±SEM	4.42E+05	1.14E+07	**	1.24E+07	NS	9.71E+06	NS

Parameter	FoxP3DTR	FoxP3DTR	Fold change	FoxP3DTR	Fold change	FoxP3DTR	Fold change
		+DTx		+DTx		+DTx
		+DTx		+GSK503		+Rescue
Lymph nodes
Total cell no.	1.18E+08	8.28E+08	7.01	3.40E+08	0.41	3.54E+08	0.43
±SEM	6.51E+06	7.53E+07	***	4.62E+07	***	4.80E+07	**
No. CD4⁺TCRb⁺	3.90E+07	2.50E+08	6.40	1.04E+08	0.42	9.90E+07	0.4
±SEM	3.11E+06	2.53E+07	***	1.85E+07	***	1.20E+07	*
No. CD8⁺TCRb⁺	3.72E+07	2.10E+08	5.66	8.94E+07	0.43	8.11E+07	0.39
±SEM	2.49E+06	1.80E+07	***	1.03E+07	***	1.25E+07	**
No. CD19⁺B220⁺	2.66E+07	1.72E+08	6.48	4.97E+07	0.29	1.40E+08	0.81
±SEM	5.30E+06	3.92E+07	**	1.23E+07	*	2.29E+07	NS
No. GR1^hiCD11b⁺	5.92E+05	1.77E+07	29.9	3.84E+06	0.22	7.65E+06	0.43
±SEM	2.14E+05	3.29E+06	***	1.04E+06	**	1.75E+06	NS
No. F4/80⁺ CD11c⁻	1.10E+06	1.11E+07	10.11	2.53E+06	0.23	6.32E+06	0.57
±SEM	3.91E+05	1.90E+06	***	9.77E+05	**	2.75E+06	NS
Spleen
Total cell no.	1.22E+08	6.41E+08	5.26	2.04E+08	0.32	2.36E+08	0.37
±SEM	1.34E+07	5.82E+07	***	3.48E+07	***	9.25E+07	**
No. CD4⁺TCRb⁺	1.90E+07	1.40E+08	7.36	3.62E+07	0.26	7.00E+07	0.5
±SEM	2.67E+06	1.45E+07	***	8.52E+06	***	2.62E+07	*
No. CD8⁺TCRb⁺	1.86E+07	1.13E+08	6.09	2.71E+07	0.24	4.94E+07	0.44
±SEM	1.61E+06	1.45E+07	***	6.61E+06	***	2.23E+07	NS
No. CD19⁺B220⁺	4.93E+07	1.09E+08	2.22	3.58E+07	0.33	6.94E+07	0.64
±SEM	9.86E+06	1.65E+07	*	1.01E+07	**	2.48E+07	NS
No. GR1^hiCD11b⁺	2.99E+06	5.95E+07	20.56	3.44E+07	0.58	2.48E+07	0.42
±SEM	1.21E+06	1.19E+07	***	5.72E+06	NS	1.14E+07	NS
No. F4/80⁺CD11c−	2.06E+06	5.57E+07	27.12	3.13E+07	0.56	2.19E+07	0.39
±SEM	4.42E+05	1.14E+07	**	1.24E+07	NS	9.71E+06	NS

Significance was determined by unpaired Student’s t test: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.