Table 1.
Gene expression in MΦ-S1PR1-KO versus WT CD11bhi TAMs
Fold changeP-valueGene symbol
2.0 0.0006 Gdpd3 
2.0 0.0191 Xpr1 
2.0 0.0064 Mterfd2 
2.0 0.0492 Nrp2 
1.6 0.0439 Rnf121 
1.6 0.0277 Inpp4b 
1.6 0.0398 Fusip1 
1.6 0.0228 Folr2 
1.5 0.0325 Aurka 
1.5 0.0336 Pptc7 
1.5 0.0461 Lmna 
1.5 0.0364 Birc5 
1.5 0.0346 Sept11 
1.5 0.0188 Nrp2 
1.5 0.0362 Baz2b 
−1.5 0.0366 Upf2 
−1.5 0.0345 Chd1 
−1.5 0.0204 Ppfia1 
−1.5 0.0102 Wdr51b 
−1.5 0.0142 Arhgap22 
−1.5 0.0097 Helb 
−1.5 0.0146 Cias1 
−1.5 0.0015 Slc35c2 
−1.5 0.0386 Mef2c 
−1.6 0.0028 Nlrp3 
−1.6 0.0473 H3f3b 
−1.6 0.0119 Agl 
−1.6 0.0004 Abcd3 
−1.6 0.0030 Slc35c2 
−1.7 0.0293 Slc44a2 
−1.7 0.0348 St3gal5 
−1.7 0.0027 Anxa3 
−1.7 0.0083 Olfml3 
−1.7 0.0033 Tnfsf9 
Fold changeP-valueGene symbol
2.0 0.0006 Gdpd3 
2.0 0.0191 Xpr1 
2.0 0.0064 Mterfd2 
2.0 0.0492 Nrp2 
1.6 0.0439 Rnf121 
1.6 0.0277 Inpp4b 
1.6 0.0398 Fusip1 
1.6 0.0228 Folr2 
1.5 0.0325 Aurka 
1.5 0.0336 Pptc7 
1.5 0.0461 Lmna 
1.5 0.0364 Birc5 
1.5 0.0346 Sept11 
1.5 0.0188 Nrp2 
1.5 0.0362 Baz2b 
−1.5 0.0366 Upf2 
−1.5 0.0345 Chd1 
−1.5 0.0204 Ppfia1 
−1.5 0.0102 Wdr51b 
−1.5 0.0142 Arhgap22 
−1.5 0.0097 Helb 
−1.5 0.0146 Cias1 
−1.5 0.0015 Slc35c2 
−1.5 0.0386 Mef2c 
−1.6 0.0028 Nlrp3 
−1.6 0.0473 H3f3b 
−1.6 0.0119 Agl 
−1.6 0.0004 Abcd3 
−1.6 0.0030 Slc35c2 
−1.7 0.0293 Slc44a2 
−1.7 0.0348 St3gal5 
−1.7 0.0027 Anxa3 
−1.7 0.0083 Olfml3 
−1.7 0.0033 Tnfsf9 

WT PyMT, S1PR1ΔMΦ PyMT, WT MCA, and S1PR1ΔMΦ MCA CD11bhi TAM (two replicates consisting of TAM from two to three individual animals for each group) were sorted by FACS and subjected to whole-genome mRNA array analysis. A common gene signature between WT and S1PR1ΔMΦ TAMs of the two tumor models is shown. P-values were calculated by two-sample t test after applying a variance filter to reduce microarray data complexity. The false discovery rate according to Benjamini and Hochberg was used to account for the multiple testing. Fold changes between the two groups of each supervised analysis were calculated for each gene.

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