| JEV peptide sequence . | Subject type . | Viruses with identical sequence . | Subset . | Variant . | Virus/BEI peptide location . |
|---|---|---|---|---|---|
| CSAVPVDW | H | DENV1 | CD8 | CSSVPVDW | MVEV |
| MTTEDMLQVW | H | CD8 | MTTEDMLRVW | JEV | |
| MTTEDMLSVW | DENV1 | ||||
| MTTEDMLTVW | DENV2,3 | ||||
| MTTEDMLKVW | DENV4 | ||||
| MTTEDMLEVW | WNV, YFV | ||||
| MTTEDMLDVW | TBEV | ||||
| ALRGLPVRY | H | ZIKV | CD8 | ALRGLPIRY | WNV, DENV2,4 |
| NLFVMDEAHFTDPASIEAHFTDPASIAARGYI | H | DENV2,3 | CD8 | NYNMIIMDEAHFTDPA | DENV1 NS3 p49 |
| IMDEAHFTDPASIARRG | DENV1 NS3 p50 | ||||
| NYNLIIMDEAHFTDPASI | DENV2 NS3 p38 | ||||
| NYNLIIMDEAHFTDPA | DENV3 NS3 p48 | ||||
| PNYNLIVMDEAHFTD | DENV4 NS3 p49 | ||||
| LIVMDEAHFTDPSSVAA | DENV4 NS3 p50 | ||||
| AHFTDPSSVAARGYIST | DENV4 NS3 p51 | ||||
| TMAMTDTTPFGQQRVFK | H | WNV | CD4 + CD8 | MVTQIAMTDTTPFGQQRa | DENV1 NS5 p60 |
| AMTDTTPFGQQRVFKEKa | DENV2 NS5 p61 | ||||
| MVTQMAMTDTTPFGQQRa | DENV3 NS5 p60 | ||||
| MVTQLAMTDTTPFGQQRa | DENV4 NS5 p60 | ||||
| GEAAAIFMT | H | WNV, DENV1,3,4, ZIKV | CD8 | MGEAAAIFMTATPPGSVb | DENV1 NS3 p54, DENV3 NS3 p52 |
| VEMGEAAAIFMTATPPGb | DENV4 NS3 p54 | ||||
| TAVLAPTRVVAAEMAEAL | H | WNV | CD8 | NVRTLILAPTRVVASEM | DENV1 NS3 p38 |
| LRTLILAPTRVVAAEM | DENV2 NS3 p30 | ||||
| TLILAPTRVVAAEMEEA | DENV3 NS3 p38, DENV4 NS3 p39 | ||||
| DVMCHATL | D | WNV, MVEV, SLEV | CD8 | DLMCHATF | DENV1–4, ZIKV |
| DAMCHATL | YFV | ||||
| AVHSDLSYWIESRYNDTW | JE | CD4 | NLAIHSDLSYWIESRL | WNV NS1 p26 | |
| WIESRYNDTWKLERAVF | JE | CD4 | RLNDTWKLERAVLGEVK | WNV NS1 p28 | |
| IESEKNETWKLARASFI | DENV NS1 p36 | ||||
| KQSVVALGSQEGGLHQAL | JE | CD4 | HATKQSVIALGSQEGALH | WNV E p34 | |
| ALGSQEGALHQALAGAI | WNV E p35 | ||||
| VVVLGSQEGAMHTALTG | DENV1 E p44 | ||||
| HAKKQDVVVLGSQEGAMH | DENV2 E p34 | ||||
| VLGSQEGAMHTALTGA | DENV2 E p35 | ||||
| HAKKQEVVVLGSQEGAMH | DENV3 E p35, DENV1b | ||||
| VLGSQEGAMHTALTGA | DENV3 E p36, | ||||
| HAKRQDVTVLGSQEGAMH | DENV4 E p36 | ||||
| VLGSQEGAMHSALAGA | DENV4 E p37 | ||||
| HGTVVIELSYSGSDGPCK | JE | CD4 | HGTVVLELQYTGTDGPCK | WNV E p44 | |
| LGKAFSTTLKGAQRLAAL | JE | CD4 | HKSGSSIGKAFTTTLKGA | WNV E p55 | |
| KAFTTTLKGAQRLAAL | WNV E p56 | ||||
| WDFGSIGGVFNSIGKAV | JE | CD4 | WDFGSVGGVFTSVGKAVH | WNV E p59 | |
| GVFNSIGKAVHQVFGGAF | VFTSVGKAVHQVFGGAFR | WNV E p60 | |||
| AISGDDCVVKPLDDRFCVVKPLDDRFATALHFL | JE | CD4 + CD8 | AVSGDDCVVKPLDDRFA | WNV NS5 p91+92c | |
| VVKPLDDRFATSLHFLNA | |||||
| ISGDDCVVKPIDDRFAT | DENV1 NS5 p115+116c | ||||
| VVKPIDDRFATALTALN | |||||
| ISGDDCVVKPLDDRFA | DENV2 NS5 p116+117c | ||||
| CVVKPLDDRFASALTAL | |||||
| MAISGDDCVVKPIDDRF | DENV3 NS5 p115+116c | ||||
| DCVVKPIDDRFANALLA | |||||
| MAISGDDCVVKPLDERF | DENV4 NS5 p116+117c | ||||
| DCVVKPLDERFSTSLLF |
| JEV peptide sequence . | Subject type . | Viruses with identical sequence . | Subset . | Variant . | Virus/BEI peptide location . |
|---|---|---|---|---|---|
| CSAVPVDW | H | DENV1 | CD8 | CSSVPVDW | MVEV |
| MTTEDMLQVW | H | CD8 | MTTEDMLRVW | JEV | |
| MTTEDMLSVW | DENV1 | ||||
| MTTEDMLTVW | DENV2,3 | ||||
| MTTEDMLKVW | DENV4 | ||||
| MTTEDMLEVW | WNV, YFV | ||||
| MTTEDMLDVW | TBEV | ||||
| ALRGLPVRY | H | ZIKV | CD8 | ALRGLPIRY | WNV, DENV2,4 |
| NLFVMDEAHFTDPASIEAHFTDPASIAARGYI | H | DENV2,3 | CD8 | NYNMIIMDEAHFTDPA | DENV1 NS3 p49 |
| IMDEAHFTDPASIARRG | DENV1 NS3 p50 | ||||
| NYNLIIMDEAHFTDPASI | DENV2 NS3 p38 | ||||
| NYNLIIMDEAHFTDPA | DENV3 NS3 p48 | ||||
| PNYNLIVMDEAHFTD | DENV4 NS3 p49 | ||||
| LIVMDEAHFTDPSSVAA | DENV4 NS3 p50 | ||||
| AHFTDPSSVAARGYIST | DENV4 NS3 p51 | ||||
| TMAMTDTTPFGQQRVFK | H | WNV | CD4 + CD8 | MVTQIAMTDTTPFGQQRa | DENV1 NS5 p60 |
| AMTDTTPFGQQRVFKEKa | DENV2 NS5 p61 | ||||
| MVTQMAMTDTTPFGQQRa | DENV3 NS5 p60 | ||||
| MVTQLAMTDTTPFGQQRa | DENV4 NS5 p60 | ||||
| GEAAAIFMT | H | WNV, DENV1,3,4, ZIKV | CD8 | MGEAAAIFMTATPPGSVb | DENV1 NS3 p54, DENV3 NS3 p52 |
| VEMGEAAAIFMTATPPGb | DENV4 NS3 p54 | ||||
| TAVLAPTRVVAAEMAEAL | H | WNV | CD8 | NVRTLILAPTRVVASEM | DENV1 NS3 p38 |
| LRTLILAPTRVVAAEM | DENV2 NS3 p30 | ||||
| TLILAPTRVVAAEMEEA | DENV3 NS3 p38, DENV4 NS3 p39 | ||||
| DVMCHATL | D | WNV, MVEV, SLEV | CD8 | DLMCHATF | DENV1–4, ZIKV |
| DAMCHATL | YFV | ||||
| AVHSDLSYWIESRYNDTW | JE | CD4 | NLAIHSDLSYWIESRL | WNV NS1 p26 | |
| WIESRYNDTWKLERAVF | JE | CD4 | RLNDTWKLERAVLGEVK | WNV NS1 p28 | |
| IESEKNETWKLARASFI | DENV NS1 p36 | ||||
| KQSVVALGSQEGGLHQAL | JE | CD4 | HATKQSVIALGSQEGALH | WNV E p34 | |
| ALGSQEGALHQALAGAI | WNV E p35 | ||||
| VVVLGSQEGAMHTALTG | DENV1 E p44 | ||||
| HAKKQDVVVLGSQEGAMH | DENV2 E p34 | ||||
| VLGSQEGAMHTALTGA | DENV2 E p35 | ||||
| HAKKQEVVVLGSQEGAMH | DENV3 E p35, DENV1b | ||||
| VLGSQEGAMHTALTGA | DENV3 E p36, | ||||
| HAKRQDVTVLGSQEGAMH | DENV4 E p36 | ||||
| VLGSQEGAMHSALAGA | DENV4 E p37 | ||||
| HGTVVIELSYSGSDGPCK | JE | CD4 | HGTVVLELQYTGTDGPCK | WNV E p44 | |
| LGKAFSTTLKGAQRLAAL | JE | CD4 | HKSGSSIGKAFTTTLKGA | WNV E p55 | |
| KAFTTTLKGAQRLAAL | WNV E p56 | ||||
| WDFGSIGGVFNSIGKAV | JE | CD4 | WDFGSVGGVFTSVGKAVH | WNV E p59 | |
| GVFNSIGKAVHQVFGGAF | VFTSVGKAVHQVFGGAFR | WNV E p60 | |||
| AISGDDCVVKPLDDRFCVVKPLDDRFATALHFL | JE | CD4 + CD8 | AVSGDDCVVKPLDDRFA | WNV NS5 p91+92c | |
| VVKPLDDRFATSLHFLNA | |||||
| ISGDDCVVKPIDDRFAT | DENV1 NS5 p115+116c | ||||
| VVKPIDDRFATALTALN | |||||
| ISGDDCVVKPLDDRFA | DENV2 NS5 p116+117c | ||||
| CVVKPLDDRFASALTAL | |||||
| MAISGDDCVVKPIDDRF | DENV3 NS5 p115+116c | ||||
| DCVVKPIDDRFANALLA | |||||
| MAISGDDCVVKPLDERF | DENV4 NS5 p116+117c | ||||
| DCVVKPLDERFSTSLLF |
JE, recovered JE patient; H, healthy JEV exposed; D, dengue exposed; MVEV, Murray Valley encephalitis virus; YFV, yellow fever virus; TBEV, tick-borne encephalitis virus; SLEV, St. Louis encephalitis virus; ZIKV, Zika virus. Variant peptides were synthesized or obtained from BEI resources; and their location within each peptide set (see Materials and methods) is given in the last column. Bold/underlined amino acids represent differences from the JEV sequence.
Likely to represent conserved epitope DTTPFGQQR (see Fig. 2 D).
Identical viral sequences, but corresponding peptides vary slightly.
Pairs of peptides tested together in the same assay.