| Genotype of donor mice | Recipients n = | Number of BM cells transplanted | Frequency of long-term reconstitutiona | Mice with MPN phenotype |
|---|---|---|---|---|
| MxCre;GFP | 19 | 20,000 | 2/19 (10%) | 0 |
| MxCre;Ezh2+/Δ;GFP | 40 | 20,000 | 13/40 (32.5%) | 1/13 (7.6%) |
| MxCre;V617F;GFP | 41 | 8,000 | 6/41 (15%) | 2/6 (33%) |
| MxCre;V617F;Ezh2+/Δ;GFP | 41 | 8,000 | 16/41 (39%) | 8/16 (50%) |
| Genotype of donor mice | Recipients | Number of BM cells transplanted | Frequency of long-term reconstitution | Mice with MPN phenotype |
|---|---|---|---|---|
| 19 | 20,000 | 2/19 (10%) | 0 | |
| 40 | 20,000 | 13/40 (32.5%) | 1/13 (7.6%) | |
| 41 | 8,000 | 6/41 (15%) | 2/6 (33%) | |
| 41 | 8,000 | 16/41 (39%) | 8/16 (50%) |
The indicated numbers of BM cells were injected into lethally irradiated recipients.
Reconstitution was scored based on the presence of >1% GFP-positive cells in the peripheral blood Gr1+ cells at 22 wk after transplantation.