Table 1. Reporter mice useful for... | Rockefeller University Press

Table 1.

Reporter mice useful for imaging myeloid cells^a

Mouse	Approach	Location	Predominant cell types labeled	Original reference	Example papers for intravital imaging of myeloid cells
Cx3cr1^GFP	Knock-In	Soluble GFP, cytosolic	Ly6C^Low Monocytes, Mac/DC > Ly6C^Hi Monocytes	Jung et al., 2000	Athero: Huo et al., 2003, Other: Geissmann et al., 2003
Cd11c^YFP	Transgenic	Soluble YFP, cytosolic	Mac/DC (YFP^hi)	Lindquist et al., 2004	Athero: Li et al., 2010 , Other: Coppieters et al., 2010
Lysm^GFP	Knock-In	Soluble GFP, cytosolic	Neutrophils > Macrophages, Monocytes	Faust et al., 2000	Athero: Drechsler et al., 2010 , Other: Egen et al., 2008
MacGreen (c-fms)	Transgenic	Cytosolic GFP	Mac/DC > monocytes neutrophils	Sasmono et al., 2003	Athero: Yu et al., 2007 Other: Looney et al., 2011
Mafia^GFP (c-fms)	IRES Transgenic	Cytosolic GFP + membrane suicide construct	Mac/DC > monocytes, neutrophils	Burnett et al., 2004	Athero: Chèvre et al., 2014, Other: Peters et al., 2008
MacBlue (c-fms)	Transgenic	Cytosolic CFP	Mac/DC > monocytes	Ovchinnikov et al., 2008	Athero: None Other: Sauter et al., 2014
Nr4a1^GFP	BAC transgenic	GFP-Cre fusion, mostly nuclear	T cells, Ly6C^Low Monocytes	Soriano, 1999	Athero: None Other: Lassailly et al., 2010
MHCII^GFP	Knock-in	Fusion Basal-intracellular Activated-surface	Antigen-presenting cells	Boes et al., 2002	Athero: None Other: Varol et al., 2009
CCR2^RFP	Knock-in	Cytosolic RFP	Ly6C^Hi monocytes	Saederup et al., 2010	Athero: None Other: Jacquelin et al., 2013

Mouse	Approach	Location	Predominant cell types labeled	Original reference	Example papers for intravital imaging of myeloid cells
Cx3cr1^GFP	Knock-In	Soluble GFP, cytosolic	Ly6C^Low Monocytes, Mac/DC > Ly6C^Hi Monocytes	Jung et al., 2000	Athero: Huo et al., 2003 Other: Geissmann et al., 2003
Cd11c^YFP	Transgenic	Soluble YFP, cytosolic	Mac/DC (YFP^hi)	Lindquist et al., 2004	Athero: Li et al., 2010 Other: Coppieters et al., 2010
Lysm^GFP	Knock-In	Soluble GFP, cytosolic	Neutrophils > Macrophages, Monocytes	Faust et al., 2000	Athero: Drechsler et al., 2010 Other: Egen et al., 2008
MacGreen (c-fms)	Transgenic	Cytosolic GFP	Mac/DC > monocytes neutrophils	Sasmono et al., 2003	Athero: Yu et al., 2007 Other: Looney et al., 2011
Mafia^GFP (c-fms)	IRES Transgenic	Cytosolic GFP + membrane suicide construct	Mac/DC > monocytes, neutrophils	Burnett et al., 2004	Athero: Chèvre et al., 2014 Other: Peters et al., 2008
MacBlue (c-fms)	Transgenic	Cytosolic CFP	Mac/DC > monocytes	Ovchinnikov et al., 2008	Athero: None Other: Sauter et al., 2014
Nr4a1^GFP	BAC transgenic	GFP-Cre fusion, mostly nuclear	T cells, Ly6C^Low Monocytes	Soriano, 1999	Athero: None Other: Lassailly et al., 2010
MHCII^GFP	Knock-in	Fusion Basal-intracellular Activated-surface	Antigen-presenting cells	Boes et al., 2002	Athero: None Other: Varol et al., 2009
CCR2^RFP	Knock-in	Cytosolic RFP	Ly6C^Hi monocytes	Saederup et al., 2010	Athero: None Other: Jacquelin et al., 2013

The genetic approach, cellular location, major cell types labeled, and examples of their use in intravital imaging of atherosclerotic plaques or other tissues are listed.