Table 2.
Analysis of productive and nonproductive VκJκ5 junctions in B and plasma cells from DH-LMP2A mice
Cell typeTotalIn-frameVPTCCPTC
 n % % % 
B cells 53 35.8 32.1 32.1 
Plasma cells 55 67.3 5.4 27.3 
Cell typeTotalIn-frameVPTCCPTC
 n % % % 
B cells 53 35.8 32.1 32.1 
Plasma cells 55 67.3 5.4 27.3 

B cells (B220pos/CD138neg) and PCs (B220neg/CD138pos) were isolated from spleens of DH-LMP2A mice and PCRs were performed on genomic DNA using Vκcons-for/Jκ5rev primers (Fig. 1 A). Productive and nonproductive VκJκ5 junctions were assigned as in-frame, VPTC, and CPTC junctions, as described in Table 1. Data are from two independent cell sorting experiments. n = total number of sequences.

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