Table 1. Analysis of productive and... | Rockefeller University Press

Table 1.

Analysis of productive and nonproductive VκJκ5 junctions in B and plasma cells from WT mice

Cell type	Total	In-frame	V^PTC	C^PTC
	n	%	%	%
Pro–B/pre–B cells	51	33.3	31.4	35.3
GC B cells	53	60.4	18.9	20.7
Plasma cells	47	72.3	2.1	25.5

Cell type	Total	In-frame	V^PTC	C^PTC
	n	%	%	%
Pro–B/pre–B cells	51	33.3	31.4	35.3
GC B cells	53	60.4	18.9	20.7
Plasma cells	47	72.3	2.1	25.5

Pro–B/pre–B cells (B220^lo/IgL^neg) and PCs (B220^neg/CD138^pos) were isolated from bone marrow and GC B cells (B220^hi/PNA^hi) from Peyer’s patches, and PCRs were performed on genomic DNA using Vκcons-for/Jκ5-rev primers (Fig. 1 A). Productive VκJκ5 junctions were assigned as in-frame and nonproductive junctions as V^PTC and C^PTC. Data are from two independent cell sorting experiments. n = total number of sequences.

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