Table 2.

TCR sequences of MR1–5-OP-RU tetramer+ cells in PLZF KO mice

Seq TRAV TRAJ CDR3α TRBV TRBJ CDR3β 
1 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-1 CASGDAEDYAEQFF 
2 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-4 CASSDRGXGSQNTLYF 
3 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-7 CASGDAEDYAEQFF 
4 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV19 TRBJ2-7 CASSIGDTYEQYF 
5 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV19 TRBJ2 CASSPTGGEGYAEQFF 
6 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ1-2 CASGDGDSYAEQFF 
7 a) TRAV1 b) TRAV6a TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-2 CASSEGRDTGQLYF 
8 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV14 TRBJ2-7 CASSFDIYEQYF 
9 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-1 CASSGTVYAEQFF 
10 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-1 CASGGTYYAEQFF 
11 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-7 CASTGTGISYEQYF 
12 TRAV1 TRAJ33 CAVRDSNYQLIW ND   
13 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-4 CASXXXGGASQNTLYF 
14 a) TRAV1 b) TRAV5Da TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-5 CASSDRGQDTQYF 
15 a) TRAV1 b) TRAV10a TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-1 CASGDGGAPSYEQYF 
16 a) TRAV1 b) TRAV10a TRAJ33 CAVRDSNYQLIW TRBV1 TRBJ2-4 CTCSAVWFSQNTLYF 

MR1–5-OP-RU tetramer+ TCR intermediate cells from PLZFnull mouse spleen, gated in a similar manner to that shown in Fig. 4 C, were sorted as single cells, and TCR-α and -β chains were sequenced. Cells were derived from spleens from two mice per group. X, sequence unclear.

a

Mixed unproductive sequences.

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