Table 1.

TCR sequences of MR1–5-OP-RU tetramer+ TCR intermediate cells

Seq TRAV TRAJ CDR3α TRBV TRBJ CDR3β 
1 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-7 CASGDGDWGREQYF 
2 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-3 CASSRXGGXSAETLYF 
3 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-5 CASSDGTXQDTQYF 
4 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ1-3 CASSGTSSGNTLYF 
5 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-3 CASGGGDSAETLYF 
6 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-3 CASSDTGGAQETLYF 
7 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV1 TRBJ2-4 CTCSGDSQNTLYF 
8 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-7 CASGVGDYEQYF 
9 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-7 CASGDGGAPSYEQYF 
10 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-3 CASGDDWGGRAETLYF 
11 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-5 CASGLGGRQDTQYF 
12 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-7 CASSAGTSSYEQYF 
13 TRAV1 TRAJ33 CAVMDSNYQLIW TRBV13-2 TRBJ2-3 CASGGDGDSAETLYF 
14 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-1 CASSKGDYAEQFF 
15 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-3 CASGEETGGAETLYF 
16 TRAV1 TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ1-2 CASSGTTNSDYTF 
17 a) TRAV1 b) TRAV6-4a TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-5 CASGLGGRQDTQYF 
18 a) TRAV1 b) TRAV6-5a TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-3 CASGDADRGQGAETLYF 
19 a) TRAV1 b) TRAV3-3a TRAJ33 CAVRDSNYQLIW TRBV13-3 TRBJ2-4 CASSDAASQNTLYF 
20 a) TRAV1 b) TRAV9Na TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBJ2-1 CASGDGDSYAEQFF 
21 a) TRAV1 b) TRAV12a TRAJ33 CAVRDSNYQLIW TRBV13-2 TRBV2-7 CXSGDGGAPSYEQYF 

MR1–5-OP-RU tetramer+ TCR-β intermediate cells from B6 mouse spleen, gated in a similar manner to that shown in Fig. 1 C, were sorted as single cells, and TCR-α and -β chains were sequenced. Data are derived from two independent experiments with spleens from two mice per experiment. X, sequence unclear.

a

Mixed unproductive sequences.

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