TCR sequences of MR1–5-OP-RU tetramer+ TCR intermediate cells
| Seq | TRAV | TRAJ | CDR3α | TRBV | TRBJ | CDR3β |
| 1 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-7 | CASGDGDWGREQYF |
| 2 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-3 | CASSRXGGXSAETLYF |
| 3 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-5 | CASSDGTXQDTQYF |
| 4 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ1-3 | CASSGTSSGNTLYF |
| 5 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGGGDSAETLYF |
| 6 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-3 | CASSDTGGAQETLYF |
| 7 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV1 | TRBJ2-4 | CTCSGDSQNTLYF |
| 8 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-7 | CASGVGDYEQYF |
| 9 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-7 | CASGDGGAPSYEQYF |
| 10 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGDDWGGRAETLYF |
| 11 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-5 | CASGLGGRQDTQYF |
| 12 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-7 | CASSAGTSSYEQYF |
| 13 | TRAV1 | TRAJ33 | CAVMDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGGDGDSAETLYF |
| 14 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-1 | CASSKGDYAEQFF |
| 15 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGEETGGAETLYF |
| 16 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ1-2 | CASSGTTNSDYTF |
| 17 | a) TRAV1 b) TRAV6-4a | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-5 | CASGLGGRQDTQYF |
| 18 | a) TRAV1 b) TRAV6-5a | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGDADRGQGAETLYF |
| 19 | a) TRAV1 b) TRAV3-3a | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-4 | CASSDAASQNTLYF |
| 20 | a) TRAV1 b) TRAV9Na | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-1 | CASGDGDSYAEQFF |
| 21 | a) TRAV1 b) TRAV12a | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBV2-7 | CXSGDGGAPSYEQYF |
| Seq | TRAV | TRAJ | CDR3α | TRBV | TRBJ | CDR3β |
| 1 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-7 | CASGDGDWGREQYF |
| 2 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-3 | CASSRXGGXSAETLYF |
| 3 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-5 | CASSDGTXQDTQYF |
| 4 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ1-3 | CASSGTSSGNTLYF |
| 5 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGGGDSAETLYF |
| 6 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-3 | CASSDTGGAQETLYF |
| 7 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV1 | TRBJ2-4 | CTCSGDSQNTLYF |
| 8 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-7 | CASGVGDYEQYF |
| 9 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-7 | CASGDGGAPSYEQYF |
| 10 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGDDWGGRAETLYF |
| 11 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-5 | CASGLGGRQDTQYF |
| 12 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-7 | CASSAGTSSYEQYF |
| 13 | TRAV1 | TRAJ33 | CAVMDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGGDGDSAETLYF |
| 14 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-1 | CASSKGDYAEQFF |
| 15 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGEETGGAETLYF |
| 16 | TRAV1 | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ1-2 | CASSGTTNSDYTF |
| 17 | a) TRAV1 b) TRAV6-4a | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-5 | CASGLGGRQDTQYF |
| 18 | a) TRAV1 b) TRAV6-5a | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-3 | CASGDADRGQGAETLYF |
| 19 | a) TRAV1 b) TRAV3-3a | TRAJ33 | CAVRDSNYQLIW | TRBV13-3 | TRBJ2-4 | CASSDAASQNTLYF |
| 20 | a) TRAV1 b) TRAV9Na | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBJ2-1 | CASGDGDSYAEQFF |
| 21 | a) TRAV1 b) TRAV12a | TRAJ33 | CAVRDSNYQLIW | TRBV13-2 | TRBV2-7 | CXSGDGGAPSYEQYF |
MR1–5-OP-RU tetramer+ TCR-β intermediate cells from B6 mouse spleen, gated in a similar manner to that shown in Fig. 1 C, were sorted as single cells, and TCR-α and -β chains were sequenced. Data are derived from two independent experiments with spleens from two mice per experiment. X, sequence unclear.
Mixed unproductive sequences.