Table 1. mOrange STOP sequencing by... | Rockefeller University Press

Table 1.

mOrange^STOP sequencing by Sanger and NGS

Sequencing method	Number of positions^a	Number of reads (mean)^b	Mutation number^c	Frequency (×10⁻⁴)^d
Sanger	222	101	31	13.8
NGS	222	87,009	22,370	11.1

Number of total cytosine in mOrange sequence.

Mean number of reads per cytosine.

Total mutation number in cytosines.

Mutation frequency calculated as c/(b × a).