Table 1.

mOrangeSTOP sequencing by Sanger and NGS

Sequencing method Number of positionsa Number of reads (mean)b Mutation numberc Frequency (×10−4)d 
Sanger 222 101 31 13.8 
NGS 222 87,009 22,370 11.1 
a

Number of total cytosine in mOrange sequence.

b

Mean number of reads per cytosine.

c

Total mutation number in cytosines.

d

Mutation frequency calculated as c/(b × a).

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