Table 2. Summary of SPR affinities and... | Rockefeller University Press

Table 2.

Summary of SPR affinities and MAIT cell activation status

MAIT TCR	TRAJ	TRBV	K_deq (μM), mean duplicate MR1–5-OP-RU	Activation by MR1–5-OP-RU	K_deq (μM), mean duplicate Ac-6-FP
Original WT (A-F7)	33	6-1	2.0 ± 0.6	+	>200
B-B10	33	6-1	4.2 ± 0. 6	ND	>200
B-C10	33	6-1	0.5 ± 0.1	ND	35.4 ± 3.0
C-A11	33	6-1	4.2 ± 0. 5	ND	>200
C-C10	33	6-1	2.2 ± 0.3	ND	>200
B-G8	33	6-1	1.4 ± 0.2	+	>200
B-F3-C1	33	6-1	9.1 ± 1.0	+	>200
#1	12	6-4	1.5 ± 0.1	+	>200
#2	12	6-4	2.2 ± 0.2	+	>200
#3	12	6-4	1.9 ± 0.2	+	>200
#1 Y95F	12	6-4	35 ± 2	-	>200
#2 Y95F	12	6-4	20 ± 1	-	>200
#3 Y95F	12	6-4	33 ± 2	-	>200
#4	20	6-4	2.4 ± 0.4	+	>200
#6	33	6-4	2.7 ± 0.7	+^a	N/D
C-F7	33	20	1.0 ± 0.3	+^a	N/D

MAIT TCR	TRAJ	TRBV	K_deq (μM), mean duplicate MR1–5-OP-RU	Activation by MR1–5-OP-RU	K_deq (μM), mean duplicate Ac-6-FP
Original WT (A-F7)	33	6-1	2.0 ± 0.6	+	>200
B-B10	33	6-1	4.2 ± 0. 6	ND	>200
B-C10	33	6-1	0.5 ± 0.1	ND	35.4 ± 3.0
C-A11	33	6-1	4.2 ± 0. 5	ND	>200
C-C10	33	6-1	2.2 ± 0.3	ND	>200
B-G8	33	6-1	1.4 ± 0.2	+	>200
B-F3-C1	33	6-1	9.1 ± 1.0	+	>200
#1	12	6-4	1.5 ± 0.1	+	>200
#2	12	6-4	2.2 ± 0.2	+	>200
#3	12	6-4	1.9 ± 0.2	+	>200
#1 Y95F	12	6-4	35 ± 2	-	>200
#2 Y95F	12	6-4	20 ± 1	-	>200
#3 Y95F	12	6-4	33 ± 2	-	>200
#4	20	6-4	2.4 ± 0.4	+	>200
#6	33	6-4	2.7 ± 0.7	+^a	N/D
C-F7	33	20	1.0 ± 0.3	+^a	N/D

5-OP-RU presented by C1R.MR1 incubated with MAIT cell–activating bacteria (Reantragoon et al., 2012) or rRL-6-CH₂OH (Patel et al., 2013).