Table 1.
Top differentially down-regulated genes in Re-stim OCA-B/Pou2af1-deficient T cells relative to WT
No.GeneDescriptionFold restimulation Ocab−/−/WT (log2)Target in combined ChIPseq?a
1 CCL1 chemokine (C-C motif) ligand 1 −9.01  
2 GZMB granzyme B −8.92  
3 IL9 interleukin 9 −8.57  
4 IFNG interferon gamma −8.49 Yes 
5 PENK Preproenkephalin −8.38  
6 IL3 interleukin 3 −7.19  
7 IL5 interleukin 5 −7.04 Yes 
8 IL22 interleukin 22 −6.43  
9 IL17A interleukin 17A −6.33  
10 CCL4 chemokine (C-C motif) ligand 4 −5.63  
11 INHBA inhibin beta-A −5.30  
12 IL2 interleukin 2 −5.00 Yes 
13 CCL3 chemokine (C-C motif) ligand 3 −4.91 Yes 
14 IL13 interleukin 13 −4.49  
15 IL24 interleukin 24 −4.49  
16 IL10 interleukin 10 −4.45 Yes 
17 LAG3 lymphocyte activation gene 3 −4.34 Yes 
18 ZBTB32 zinc finger and BTB domain containing 32 −4.16 Yes 
19 CXCL2 chemokine (C-X-C motif) ligand 2 −4.16  
20 CRABP2 cellular retinoic acid binding protein II −4.09 Yes 
21 IL6 interleukin 6 −4.06  
22 TIGIT T cell immunoreceptor with Ig and ITIM domains −3.70  
23 TIMP1 tissue inhibitor of metalloproteinase 1 −3.65  
24 RGS1 regulator of G-protein signaling 1 −3.56 Yes 
25 ERO1L ERO1-like (Saccharomyces cerevisiae) −3.56  
26 0610010O12RIK RIKEN cDNA 0610010O12 gene −3.52  
27 SERPINB6B serine (or cysteine) peptidase inhibitor, clade B, member 6b −3.50 Yes 
28 CSF2 colony stimulating factor 2 (granulocyte-macrophage) −3.34 Yes 
29 IL4 interleukin 4 −3.33  
30 TNFRSF4 (OX40) tumor necrosis factor receptor superfamily, member 4 −3.22 Yes 
31 SLAMF7 SLAM family member 7 −3.21 Yes 
32 CCL7 chemokine (C-C motif) ligand 7 −3.20  
33 IL21 interleukin 21 −3.18 Yes 
34 EGLN3 EGL nine homolog 3 (Caenorhabditis elegans) −3.17 Yes 
35 CCL20 chemokine (C-C motif) ligand 20 −3.12  
36 CISH cytokine inducible SH2 containing protein −3.06 Yes 
37 IL17F interleukin 17F −3.06  
38 IL31 interleukin 31 −3.00 Yes 
39 IL2RA (CD25) interleukin 2 receptor, alpha chain −2.96 Yes 
40 SERPINB9 serine (or cysteine) peptidase inhibitor, clade B, member 9 −2.95 Yes 
41 OSM oncostatin M −2.93 Yes 
42 BATF basic leucine zipper transcription factor, ATF-like −2.89 Yes 
43 FAM132A family with sequence similarity 132, member A −2.77  
44 ASNS asparagine synthetase −2.73  
45 DDT d-dopachrome tautomerase −2.71  
46 WISP2 WNT1 inducible signaling pathway protein 2 −2.69  
47 CASP6 caspase 6 −2.65  
48 TNFSF8 tumor necrosis factor (ligand) superfamily, member 8 −2.59 Yes 
49 CXCL3 chemokine (C-X-C motif) ligand 3 −2.56  
50 IL27 interleukin 27 −2.52  
51 HEMK1 HemK methyltransferase family member 1 −2.49 Yes 
52 CD200 CD200 antigen −2.42 Yes 
53 BC048355 cDNA sequence BC048355 −2.43  
54 NRN1 neuritin 1 −2.42 Yes 
55 CTLA4 (CD152) Cytotoxic T lymphocyte associated protein 4 −2.39 Yes 
56 TBX21 (TBET) T-box 21 −2.37 Yes 
57 AGPAT4 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) −2.21  
58 LRMP lymphoid-restricted membrane protein −2.21 Yes 
59 CD70 (TNFSF7) CD70 antigen −2.18  
60 XCL1 chemokine (C motif) ligand 1 −2.17  
61 0610007P14RIK RIKEN cDNA 0610007P14 gene −2.13 Yes 
62 BATF2 basic leucine zipper transcription factor, ATF-like 2 −2.12  
63 ACOT7 acyl-CoA thioesterase 7 −2.08 Yes 
64 ARHGDIG Rho GDP dissociation inhibitor (GDI) gamma −1.99  
65 CCR8 chemokine (C-C motif) receptor 8 −1.97 Yes 
66 ABHD14A abhydrolase domain containing 14A −1.96  
67 TNFSF14 tumor necrosis factor (ligand) superfamily, member 14 −1.94  
68 HBEGF heparin-binding EGF-like growth factor −1.91  
69 STC2 stanniocalcin 2 −1.89  
70 ZEB2 zinc finger E-box binding homeobox 2 −1.86  
71 PLA2G12A phospholipase A2, group XIIA −1.86  
72 TNIP3 TNFAIP3 interacting protein 3 −1.78  
73 ACY1 aminoacylase 1 −1.75  
74 GSTT2 glutathione S-transferase, theta 2 −1.74 Yes 
75 SPRR2E small proline rich protein 2E −1.72  
76 STAT5A signal transducer and activator of transcription 5A −1.70 Yes 
77 GALK1 galactokinase 1 −1.68 Yes 
78 SDF4 stromal cell–derived factor 4 −1.68 Yes 
79 MRPS2 mitochondrial ribosomal protein S2 −1.65  
80 TIMM10 Translocase of inner mitochondrial membrane 10 −1.62  
81 IL12RB1 interleukin 12 receptor, beta 1 −1.60 Yes 
82 SLC13A3 solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3 −1.60  
83 ST6GALNAC4 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 −1.58  
84 RBPSUH-RS3 recombining binding protein suppressor of hairless (Drosophila), related sequence 3 −1.56  
85 WISP1 WNT1 inducible signaling pathway protein 1 −1.56 Yes 
86 ETV6 ets variant gene 6 (TEL oncogene) −1.55 Yes 
87 POLR3G polymerase (RNA) III (DNA directed) polypeptide G −1.55  
88 AREG amphiregulin −1.54  
89 HIRIP3 HIRA interacting protein 3 −1.52 Yes 
90 TLCD1 TLC domain containing 1 −1.52 Yes 
91 PLAGL2 pleomorphic adenoma gene-like 2 −1.51 Yes 
92 NDRG1 N-myc downstream regulated gene 1 −1.50  
93 ICOS inducible T cell co-stimulator −1.50 Yes 
94 MYC myelocytomatosis oncogene −1.38 Yes 
95 CCND2 cyclin D2 −1.37 Yes 
96 RBPJ recombination signal binding protein for immunoglobulin kappa J region −1.36 Yes 
97 MIF macrophage migration inhibitory factor −1.29  
98 ELL2 elongation factor RNA polymerase II 2 −1.28  
99 PPA1 pyrophosphatase (inorganic) 1 −1.24  
100 COQ4 coenzyme Q4 homolog (yeast) −1.17  
101 LAP3 leucine aminopeptidase 3 −1.17  
201 GADD45B growth arrest and DNA damage-inducible 45 beta −1.13 Yes 
103 ECSCR endothelial cell-specific chemotaxis regulator −1.13  
104 CHCHD4 coiled-coil-helix-coiled-coil-helix domain containing 4 −1.09 Yes 
105 ATF3 activating transcription factor 3 −1.07 Yes 
106 LAD1 ladinin −1.04  
107 TMEM97 transmembrane protein 97 −1.03  
108 HSPA5 heat shock protein 5 −1.02 Yes 
109 YWHAG tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide −1.02 Yes 
110 1810029B16RIK RIKEN cDNA 1810029B16 gene −1.01  
No.GeneDescriptionFold restimulation Ocab−/−/WT (log2)Target in combined ChIPseq?a
1 CCL1 chemokine (C-C motif) ligand 1 −9.01  
2 GZMB granzyme B −8.92  
3 IL9 interleukin 9 −8.57  
4 IFNG interferon gamma −8.49 Yes 
5 PENK Preproenkephalin −8.38  
6 IL3 interleukin 3 −7.19  
7 IL5 interleukin 5 −7.04 Yes 
8 IL22 interleukin 22 −6.43  
9 IL17A interleukin 17A −6.33  
10 CCL4 chemokine (C-C motif) ligand 4 −5.63  
11 INHBA inhibin beta-A −5.30  
12 IL2 interleukin 2 −5.00 Yes 
13 CCL3 chemokine (C-C motif) ligand 3 −4.91 Yes 
14 IL13 interleukin 13 −4.49  
15 IL24 interleukin 24 −4.49  
16 IL10 interleukin 10 −4.45 Yes 
17 LAG3 lymphocyte activation gene 3 −4.34 Yes 
18 ZBTB32 zinc finger and BTB domain containing 32 −4.16 Yes 
19 CXCL2 chemokine (C-X-C motif) ligand 2 −4.16  
20 CRABP2 cellular retinoic acid binding protein II −4.09 Yes 
21 IL6 interleukin 6 −4.06  
22 TIGIT T cell immunoreceptor with Ig and ITIM domains −3.70  
23 TIMP1 tissue inhibitor of metalloproteinase 1 −3.65  
24 RGS1 regulator of G-protein signaling 1 −3.56 Yes 
25 ERO1L ERO1-like (Saccharomyces cerevisiae) −3.56  
26 0610010O12RIK RIKEN cDNA 0610010O12 gene −3.52  
27 SERPINB6B serine (or cysteine) peptidase inhibitor, clade B, member 6b −3.50 Yes 
28 CSF2 colony stimulating factor 2 (granulocyte-macrophage) −3.34 Yes 
29 IL4 interleukin 4 −3.33  
30 TNFRSF4 (OX40) tumor necrosis factor receptor superfamily, member 4 −3.22 Yes 
31 SLAMF7 SLAM family member 7 −3.21 Yes 
32 CCL7 chemokine (C-C motif) ligand 7 −3.20  
33 IL21 interleukin 21 −3.18 Yes 
34 EGLN3 EGL nine homolog 3 (Caenorhabditis elegans) −3.17 Yes 
35 CCL20 chemokine (C-C motif) ligand 20 −3.12  
36 CISH cytokine inducible SH2 containing protein −3.06 Yes 
37 IL17F interleukin 17F −3.06  
38 IL31 interleukin 31 −3.00 Yes 
39 IL2RA (CD25) interleukin 2 receptor, alpha chain −2.96 Yes 
40 SERPINB9 serine (or cysteine) peptidase inhibitor, clade B, member 9 −2.95 Yes 
41 OSM oncostatin M −2.93 Yes 
42 BATF basic leucine zipper transcription factor, ATF-like −2.89 Yes 
43 FAM132A family with sequence similarity 132, member A −2.77  
44 ASNS asparagine synthetase −2.73  
45 DDT d-dopachrome tautomerase −2.71  
46 WISP2 WNT1 inducible signaling pathway protein 2 −2.69  
47 CASP6 caspase 6 −2.65  
48 TNFSF8 tumor necrosis factor (ligand) superfamily, member 8 −2.59 Yes 
49 CXCL3 chemokine (C-X-C motif) ligand 3 −2.56  
50 IL27 interleukin 27 −2.52  
51 HEMK1 HemK methyltransferase family member 1 −2.49 Yes 
52 CD200 CD200 antigen −2.42 Yes 
53 BC048355 cDNA sequence BC048355 −2.43  
54 NRN1 neuritin 1 −2.42 Yes 
55 CTLA4 (CD152) Cytotoxic T lymphocyte associated protein 4 −2.39 Yes 
56 TBX21 (TBET) T-box 21 −2.37 Yes 
57 AGPAT4 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) −2.21  
58 LRMP lymphoid-restricted membrane protein −2.21 Yes 
59 CD70 (TNFSF7) CD70 antigen −2.18  
60 XCL1 chemokine (C motif) ligand 1 −2.17  
61 0610007P14RIK RIKEN cDNA 0610007P14 gene −2.13 Yes 
62 BATF2 basic leucine zipper transcription factor, ATF-like 2 −2.12  
63 ACOT7 acyl-CoA thioesterase 7 −2.08 Yes 
64 ARHGDIG Rho GDP dissociation inhibitor (GDI) gamma −1.99  
65 CCR8 chemokine (C-C motif) receptor 8 −1.97 Yes 
66 ABHD14A abhydrolase domain containing 14A −1.96  
67 TNFSF14 tumor necrosis factor (ligand) superfamily, member 14 −1.94  
68 HBEGF heparin-binding EGF-like growth factor −1.91  
69 STC2 stanniocalcin 2 −1.89  
70 ZEB2 zinc finger E-box binding homeobox 2 −1.86  
71 PLA2G12A phospholipase A2, group XIIA −1.86  
72 TNIP3 TNFAIP3 interacting protein 3 −1.78  
73 ACY1 aminoacylase 1 −1.75  
74 GSTT2 glutathione S-transferase, theta 2 −1.74 Yes 
75 SPRR2E small proline rich protein 2E −1.72  
76 STAT5A signal transducer and activator of transcription 5A −1.70 Yes 
77 GALK1 galactokinase 1 −1.68 Yes 
78 SDF4 stromal cell–derived factor 4 −1.68 Yes 
79 MRPS2 mitochondrial ribosomal protein S2 −1.65  
80 TIMM10 Translocase of inner mitochondrial membrane 10 −1.62  
81 IL12RB1 interleukin 12 receptor, beta 1 −1.60 Yes 
82 SLC13A3 solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3 −1.60  
83 ST6GALNAC4 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 −1.58  
84 RBPSUH-RS3 recombining binding protein suppressor of hairless (Drosophila), related sequence 3 −1.56  
85 WISP1 WNT1 inducible signaling pathway protein 1 −1.56 Yes 
86 ETV6 ets variant gene 6 (TEL oncogene) −1.55 Yes 
87 POLR3G polymerase (RNA) III (DNA directed) polypeptide G −1.55  
88 AREG amphiregulin −1.54  
89 HIRIP3 HIRA interacting protein 3 −1.52 Yes 
90 TLCD1 TLC domain containing 1 −1.52 Yes 
91 PLAGL2 pleomorphic adenoma gene-like 2 −1.51 Yes 
92 NDRG1 N-myc downstream regulated gene 1 −1.50  
93 ICOS inducible T cell co-stimulator −1.50 Yes 
94 MYC myelocytomatosis oncogene −1.38 Yes 
95 CCND2 cyclin D2 −1.37 Yes 
96 RBPJ recombination signal binding protein for immunoglobulin kappa J region −1.36 Yes 
97 MIF macrophage migration inhibitory factor −1.29  
98 ELL2 elongation factor RNA polymerase II 2 −1.28  
99 PPA1 pyrophosphatase (inorganic) 1 −1.24  
100 COQ4 coenzyme Q4 homolog (yeast) −1.17  
101 LAP3 leucine aminopeptidase 3 −1.17  
201 GADD45B growth arrest and DNA damage-inducible 45 beta −1.13 Yes 
103 ECSCR endothelial cell-specific chemotaxis regulator −1.13  
104 CHCHD4 coiled-coil-helix-coiled-coil-helix domain containing 4 −1.09 Yes 
105 ATF3 activating transcription factor 3 −1.07 Yes 
106 LAD1 ladinin −1.04  
107 TMEM97 transmembrane protein 97 −1.03  
108 HSPA5 heat shock protein 5 −1.02 Yes 
109 YWHAG tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide −1.02 Yes 
110 1810029B16RIK RIKEN cDNA 1810029B16 gene −1.01  

Included are genes normally induced by twofold or greater upon restimulation, showing twofold or greater down-regulation in the absence of OCA-B. See Table S2 for full dataset. Empty cells in the last column indicate “No,” although some genes may have just missed the arbitrary threshold and would be false negatives.

a

Merged data for ChIPseq binding of Oct1 and OCA-B in rested and restimulated T cells.

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