Secondary recipients were transplanted with 50, 500, or 5,000 LK cells of the indicated genotypes sorted from primary recipients. Number of mice (out of total injected) that showed engraftment of CD45.2⁺ cells in the peripheral blood at week 9 (n = 6–15 per group) are listed. All calculations and statistical analyses were done using the ELDA algorithm (Hu and Smyth, 2009). Three independent experiments were performed.