Table 1. LDA Group Number of cells... | Rockefeller University Press

Table 1.

LDA

Group	Number of cells transplanted	Number of reconstituted mice/number of primary recipients	HSC frequency (95% CI)
Control	1,000	3/10	1 in 26,559 (54,453–12,954)
Control	10,000	4/10
Control	100,000	9/10
GzmB^−/−	1,000	2/10	1 in 5,354 (10,164–2,820)
GzmB^−/−	10,000	10/12	1 in 5,354 (10,164–2,820)

Group	Number of cells transplanted	Number of reconstituted mice/number of primary recipients	HSC frequency (95% CI)
Control	1,000	3/10	1 in 26,559 (54,453–12,954)
Control	10,000	4/10
Control	100,000	9/10
GzmB^−/−	1,000	2/10	1 in 5,354 (10,164–2,820)
GzmB^−/−	10,000	10/12	1 in 5,354 (10,164–2,820)

Serially diluted control or GzmB^−/− total BM cells were transplanted with supportive WT total BM cells, and HSC engraftment was quantified 14 wk after transplantation. Frequency of HSCs was calculated, according to Poisson statistics using ELDA software, from combined results of two independent experiments with n = 10/group (two-tailed Student’s t test; P = 0.026). Positive: >1% engraftment BM (LSK SLAM). CI, confidence interval.

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