LDA
| Group | Number of cells transplanted | Number of reconstituted mice/number of primary recipients | HSC frequency (95% CI) |
| Control | 1,000 | 3/10 | 1 in 26,559 (54,453–12,954) |
| Control | 10,000 | 4/10 | |
| Control | 100,000 | 9/10 | |
| GzmB−/− | 1,000 | 2/10 | 1 in 5,354 (10,164–2,820) |
| GzmB−/− | 10,000 | 10/12 |
| Group | Number of cells transplanted | Number of reconstituted mice/number of primary recipients | HSC frequency (95% CI) |
| Control | 1,000 | 3/10 | 1 in 26,559 (54,453–12,954) |
| Control | 10,000 | 4/10 | |
| Control | 100,000 | 9/10 | |
| GzmB−/− | 1,000 | 2/10 | 1 in 5,354 (10,164–2,820) |
| GzmB−/− | 10,000 | 10/12 |
Serially diluted control or GzmB−/− total BM cells were transplanted with supportive WT total BM cells, and HSC engraftment was quantified 14 wk after transplantation. Frequency of HSCs was calculated, according to Poisson statistics using ELDA software, from combined results of two independent experiments with n = 10/group (two-tailed Student’s t test; P = 0.026). Positive: >1% engraftment BM (LSK SLAM). CI, confidence interval.