Vagotomy shifts to proinflammatory lipid mediators-metabololipidomics
| Q1 | Q3 | Inflam. time = 4 h | Vagotomy + Inflam. time = 4 h | |||||
| Average | SEM | Average | SEM | |||||
| pg/exudate | pg/exudate | |||||||
| DHA bioactive metabolome | ||||||||
| RvD1 | 375 | 215 | 27.7 | ± | 3.8 | 8.5 | ± | 2.1 |
| RvD2 | 375 | 141 | * | * | ||||
| RvD3 | 375 | 147 | * | * | ||||
| RvD5 | 359 | 199 | 26.4 | ± | 5.7 | 60.1 | ± | 6.9 |
| RvD6 | 359 | 159 | 4.8 | ± | 1.2 | 5.9 | ± | 2.2 |
| 4,14-diHDHA | 359 | 101 | 10.5 | ± | 1.9 | 30.1 | ± | 3.2 |
| MaR1 | 359 | 221 | 7.2 | ± | 2.1 | 9.0 | ± | 2.9 |
| PD1 | 359 | 153 | 38.7 | ± | 8.2 | 90.5 | ± | 7.7 |
| 20-OH-PD1 | 359 | 153 | * | * | ||||
| 4-HDHA | 343 | 101 | 151.8 | ± | 54.7 | 104.1 | ± | 16.9 |
| 7-HDHA | 343 | 141 | 24.9 | ± | 6.9 | 21.9 | ± | 5.1 |
| 14-HDHA | 343 | 205 | 10785.8 | ± | 3675.5 | 7852.7 | ± | 4296.4 |
| 17-HDHA | 343 | 245 | 1249.6 | ± | 473.2 | 2859.1 | ± | 121.8 |
| DHA | 327 | 283 | 19793.0 | ± | 7673.2 | 18514.9 | ± | 689.9 |
| AA bioactive metabolome | ||||||||
| LXB4 | 351 | 115 | 4.7 | ± | 2.4 | 4.2 | ± | 0.7 |
| LXB4 | 351 | 221 | 43.2 | ± | 18.5 | 60.6 | ± | 13.9 |
| 5,15-diHETE | 335 | 115 | 82.4 | ± | 13.9 | 246.7 | ± | 4.7 |
| LTB4 | 335 | 195 | 24.1 | ± | 5.7 | 113.8 | ± | 30.4 |
| 20-OH-LTB4 | 351 | 195 | 4.4 | ± | 1.2 | 6.2 | ± | 2.7 |
| PGD2 | 351 | 189 | 79.1 | ± | 35.4 | 84.6 | ± | 7.9 |
| PGE2 | 351 | 233 | 198.3 | ± | 19.6 | 295.0 | ± | 67.4 |
| PGF2α | 353 | 193 | 86.9 | ± | 13.5 | 76.2 | ± | 19.9 |
| TXB2 | 369 | 169 | 133.3 | ± | 9.4 | 266.5 | ± | 32.9 |
| 5-HETE | 319 | 115 | 368.9 | ± | 92.9 | 364.1 | ± | 95.0 |
| 12-HETE | 319 | 179 | 14681.2 | ± | 3618.1 | 11572.2 | ± | 4095.4 |
| 15-HETE | 319 | 219 | 857.2 | ± | 202.8 | 660.4 | ± | 212.5 |
| AA | 303 | 259 | 38.8 | ± | 7.2 | 26.2 | ± | 4.7 |
| EPA bioactive metabolome | ||||||||
| RvE1 | 349 | 195 | * | * | ||||
| RvE2 | 333 | 199 | * | * | ||||
| LXA5 | 349 | 115 | * | * | ||||
| LXB5 | 349 | 221 | * | * | ||||
| 5,15-diHEPE | 333 | 115 | * | * | ||||
| 5-HEPE | 317 | 115 | 20.2 | ± | 4.5 | 12.8 | ± | 1.7 |
| 12-HEPE | 317 | 179 | 1176.1 | ± | 147.1 | 801.9 | ± | 116.0 |
| 18-HEPE | 317 | 259 | 694.9 | ± | 66.2 | 354.6 | ± | 124.0 |
| 15-HEPE | 317 | 219 | 16.3 | ± | 4.5 | 17.9 | ± | 2.4 |
| EPA | 301 | 257 | 3.5 | ± | 2.5 | 8.6 | ± | 0.7 |
| Q1 | Q3 | Inflam. time = 4 h | Vagotomy + Inflam. time = 4 h | |||||
| Average | SEM | Average | SEM | |||||
| pg/exudate | pg/exudate | |||||||
| DHA bioactive metabolome | ||||||||
| RvD1 | 375 | 215 | 27.7 | ± | 3.8 | 8.5 | ± | 2.1 |
| RvD2 | 375 | 141 | * | * | ||||
| RvD3 | 375 | 147 | * | * | ||||
| RvD5 | 359 | 199 | 26.4 | ± | 5.7 | 60.1 | ± | 6.9 |
| RvD6 | 359 | 159 | 4.8 | ± | 1.2 | 5.9 | ± | 2.2 |
| 4,14-diHDHA | 359 | 101 | 10.5 | ± | 1.9 | 30.1 | ± | 3.2 |
| MaR1 | 359 | 221 | 7.2 | ± | 2.1 | 9.0 | ± | 2.9 |
| PD1 | 359 | 153 | 38.7 | ± | 8.2 | 90.5 | ± | 7.7 |
| 20-OH-PD1 | 359 | 153 | * | * | ||||
| 4-HDHA | 343 | 101 | 151.8 | ± | 54.7 | 104.1 | ± | 16.9 |
| 7-HDHA | 343 | 141 | 24.9 | ± | 6.9 | 21.9 | ± | 5.1 |
| 14-HDHA | 343 | 205 | 10785.8 | ± | 3675.5 | 7852.7 | ± | 4296.4 |
| 17-HDHA | 343 | 245 | 1249.6 | ± | 473.2 | 2859.1 | ± | 121.8 |
| DHA | 327 | 283 | 19793.0 | ± | 7673.2 | 18514.9 | ± | 689.9 |
| AA bioactive metabolome | ||||||||
| LXB4 | 351 | 115 | 4.7 | ± | 2.4 | 4.2 | ± | 0.7 |
| LXB4 | 351 | 221 | 43.2 | ± | 18.5 | 60.6 | ± | 13.9 |
| 5,15-diHETE | 335 | 115 | 82.4 | ± | 13.9 | 246.7 | ± | 4.7 |
| LTB4 | 335 | 195 | 24.1 | ± | 5.7 | 113.8 | ± | 30.4 |
| 20-OH-LTB4 | 351 | 195 | 4.4 | ± | 1.2 | 6.2 | ± | 2.7 |
| PGD2 | 351 | 189 | 79.1 | ± | 35.4 | 84.6 | ± | 7.9 |
| PGE2 | 351 | 233 | 198.3 | ± | 19.6 | 295.0 | ± | 67.4 |
| PGF2α | 353 | 193 | 86.9 | ± | 13.5 | 76.2 | ± | 19.9 |
| TXB2 | 369 | 169 | 133.3 | ± | 9.4 | 266.5 | ± | 32.9 |
| 5-HETE | 319 | 115 | 368.9 | ± | 92.9 | 364.1 | ± | 95.0 |
| 12-HETE | 319 | 179 | 14681.2 | ± | 3618.1 | 11572.2 | ± | 4095.4 |
| 15-HETE | 319 | 219 | 857.2 | ± | 202.8 | 660.4 | ± | 212.5 |
| AA | 303 | 259 | 38.8 | ± | 7.2 | 26.2 | ± | 4.7 |
| EPA bioactive metabolome | ||||||||
| RvE1 | 349 | 195 | * | * | ||||
| RvE2 | 333 | 199 | * | * | ||||
| LXA5 | 349 | 115 | * | * | ||||
| LXB5 | 349 | 221 | * | * | ||||
| 5,15-diHEPE | 333 | 115 | * | * | ||||
| 5-HEPE | 317 | 115 | 20.2 | ± | 4.5 | 12.8 | ± | 1.7 |
| 12-HEPE | 317 | 179 | 1176.1 | ± | 147.1 | 801.9 | ± | 116.0 |
| 18-HEPE | 317 | 259 | 694.9 | ± | 66.2 | 354.6 | ± | 124.0 |
| 15-HEPE | 317 | 219 | 16.3 | ± | 4.5 | 17.9 | ± | 2.4 |
| EPA | 301 | 257 | 3.5 | ± | 2.5 | 8.6 | ± | 0.7 |
Individual lipid mediators and precursor/pathway markers at: Q1, M-H (parent ion); and Q3, diagnostic ion in the MS/MS (daughter ion) along with mean ± SEM values for each of the mediators (pg/exudate) identified in the 4-h exudates. Detection limit, ∼1 pg. *, below limits.