Global analysis of unique mutation frequencies of the JH4 intronic region
| JH4 intronic region | WT | Fanca−/− | P-value |
| Number of mice | 5 | 5 | |
| Mutated sequences analyzed | 150 | 161 | |
| Unique mutations (total) | 787 | 665 | |
| Overall mutation frequency | 10.5 x 10−2 | 8.8 x 10−2 | 0.0008 |
| A/T mutations | 11.2 x 10−2 | 8.7 x 10−2 | 0.0001 |
| Transversions | 9.1 x 10−2 | 8.2 x 10−2 | 0.2637 |
| Transitions | 15.5 x 10−2 | 9.7 x 10−2 | <10−5 |
| Motif | |||
| WA | 33.7 x 10−2 | 19.7 x 10−2 | <10−4 |
| TW | 14.7 x 10−2 | 9.5 x 10−2 | 0.1637 |
| G/C mutations | 9.4 x 10−2 | 9.0 x 10−2 | 0.6013 |
| Transversions | 6.9 x 10−2 | 6.4 x 10−2 | 0.5355 |
| Transitions | 14.5 x 10−2 | 14.3 x 10−2 | 0.9504 |
| Motif | |||
| WRC | 53.3 x 10−2 | 44.0 x 10−2 | 0.327 |
| GYW | 36.8 x 10−2 | 36.8 x 10−2 | 1.0 |
| JH4 intronic region | WT | Fanca−/− | P-value |
| Number of mice | 5 | 5 | |
| Mutated sequences analyzed | 150 | 161 | |
| Unique mutations (total) | 787 | 665 | |
| Overall mutation frequency | 10.5 x 10−2 | 8.8 x 10−2 | 0.0008 |
| A/T mutations | 11.2 x 10−2 | 8.7 x 10−2 | 0.0001 |
| Transversions | 9.1 x 10−2 | 8.2 x 10−2 | 0.2637 |
| Transitions | 15.5 x 10−2 | 9.7 x 10−2 | <10−5 |
| Motif | |||
| WA | 33.7 x 10−2 | 19.7 x 10−2 | <10−4 |
| TW | 14.7 x 10−2 | 9.5 x 10−2 | 0.1637 |
| G/C mutations | 9.4 x 10−2 | 9.0 x 10−2 | 0.6013 |
| Transversions | 6.9 x 10−2 | 6.4 x 10−2 | 0.5355 |
| Transitions | 14.5 x 10−2 | 14.3 x 10−2 | 0.9504 |
| Motif | |||
| WRC | 53.3 x 10−2 | 44.0 x 10−2 | 0.327 |
| GYW | 36.8 x 10−2 | 36.8 x 10−2 | 1.0 |
All mutation frequencies were calculated according to the SHMTool algorithm. Bold denotes a significant decrease in the mutation frequency compared with WT. Underlining indicates the mutated residues in the motifs.