Table 1. Features of the murine ETP-ALLs... | Rockefeller University Press

Table 1.

Features of the murine ETP-ALLs observed in transplanted recipients

Mouse ID	Vector	Latency (days)	Peripheral WBC/µl (×10³)	Immunophenotype by flow cytometry^a	^bImmuno-histochemistry	Pathology
647	Il7r-IL241-242TC-GFP	69	ND	ND	GFP⁺,IC-Cd3⁺,Mpo⁺	Splenomegaly (spleen = 0.73 g). Leukemic infiltrates in brain, kidney, and liver
649	Il7r-IL241-242TC-GFP	69	ND	ND	ND	Splenomegaly (spleen = 0.55 g). Animal was found dead and tissues were partially autolysed. Leukemic infiltrates in BM, brain, liver, and spleen.
650	Il7r-IL241-242TC-GFP	64	91	Gr1⁺Mac1⁺GFP⁺	GFP⁺, Mpo⁺	Splenomegaly (spleen = 0.8 g). Leukemic infiltrates in brain, kidney, liver, and lung.
651	Il7r-IL241-242TC-GFP	81	29.4	Gr1⁺Mac1⁺GFP⁺	IC-Cd3⁺, Mpo⁺, Cd117 low	Splenomegaly (spleen = 0.88 g). Leukemic infiltrates in liver and lung. Secondary transplants developed tumors with the same immunophenotype at 24 d.
652	Il7r-IL241-242TC-GFP	78	64.78	Gr1⁺Mac1⁺GFP⁺	IC-Cd3⁺, Cd117 low	Splenomegaly (spleen = 0.73 g). Leukemic infiltrates in brain, eye, kidney, liver, and lung.
653	Il7r-IL241-242TC-GFP	74	33.72	Gr1⁺Mac1⁺GFP⁺	GFP⁺, IC-Cd3⁺, Mpo⁺	Splenomegaly (spleen = 1.25 g). Leukemic infiltrates in brain, kidney, liver, and lung.
655	Il7r-GcinsL243-GFP	71	30.76	Sca1⁺Cd71⁺GFP⁺	GFP⁺, IC-Cd3⁺, Gata1⁺, Runx1⁺, Ly6b⁺, TdT⁺ Cd34⁺	Splenomegaly (spleen = 0.96 g). Leukemic infiltrates in liver, lung, focally in brain/meninges.
656^c	Il7r-GcinsL243-GFP	78	32	Cd117⁺Cd71⁺GFP⁺	Pop1: IC-Cd3⁺, Runx1⁺, Pop2: IC-Cd3⁺, Runx1⁺ and Gata-1⁺	Splenomegaly (spleen = 0.77 g). Leukemic infiltrates in liver and lung.
657	Il7r-GcinsL243-GFP	43	49.46	Sca1⁺Cd71⁺GFP⁺	IC-Cd3⁺, Gata1⁺, Runx1⁺, Ly6b⁺, TdT⁺, Cd117⁺, Fe/80⁺, Mac2⁺	Splenomegaly (spleen = 0.88 g). Leukemic infiltrates in liver, lung, focally in brain/meninges.
658^c	Il7r-GcinsL243-GFP	96	99.9	Cd71⁺GFP⁺	Pop1: IC-Cd3⁺, Runx1⁺, Pop2: IC-Cd3+, Runx1⁺ and Gata-1⁺	Splenomegaly (spleen = 0.9 g). Leukemic infiltrates in liver and lung. Secondary transplants developed identical tumors at 24 d.
659	Il7r-GcinsL243-GFP	71	48.89	Cd4⁺Cd8⁺GFP⁺	IC-Cd3⁺Tdt⁺	The only T-ALL case. Splenomegaly (spleen = 0.54 g). Enlarged thymus (thymus = 0.132 g). Leukemic infiltrates in liver, lung, brain, and kidney.
242^d	Lmo2-mCherry	89	44.3	mCherry⁺Gr1⁺Mac1⁺	IC-Cd3⁺Mpo⁺	All animals had splenomegaly (range: spleen = 0.258–1.163 g). Enlarged thymus (range: thymus = 0.064–0.116 g). Leukemic infiltrates in lungs, liver, lymph nodes, and meninges.
243^d, 244^d, 245^d	Lmo2-mCherry	71	195-528	mCherry⁺Gr1⁺Mac1⁺
611	Lmo2-mCherry	154	36.18	Cd117⁺, Cd71⁺, B220⁺, mCherry⁺	Cd117 weakly +	Splenomegaly (spleen = 0.385 g). Leukemic infiltrates in lung, spleen, liver, BM, and meninges.
612	Lmo2-mCherry	111	20.42	Gr1⁺, Mac1⁺, Cd117⁺, Cd71⁺, Cd5^low, mCherry⁺	Cd117⁺, Cd43⁺	Splenomegaly (spleen = 0.825 g). Leukemic infiltrates in liver, spleen, and kidney.

Mouse ID	Vector	Latency (days)	Peripheral WBC/µl (×10³)	Immunophenotype by flow cytometry^a	^bImmuno-histochemistry	Pathology
647	Il7r-IL241-242TC-GFP	69	ND	ND	GFP⁺,IC-Cd3⁺,Mpo⁺	Splenomegaly (spleen = 0.73 g). Leukemic infiltrates in brain, kidney, and liver
649	Il7r-IL241-242TC-GFP	69	ND	ND	ND	Splenomegaly (spleen = 0.55 g). Animal was found dead and tissues were partially autolysed. Leukemic infiltrates in BM, brain, liver, and spleen.
650	Il7r-IL241-242TC-GFP	64	91	Gr1⁺Mac1⁺GFP⁺	GFP⁺, Mpo⁺	Splenomegaly (spleen = 0.8 g). Leukemic infiltrates in brain, kidney, liver, and lung.
651	Il7r-IL241-242TC-GFP	81	29.4	Gr1⁺Mac1⁺GFP⁺	IC-Cd3⁺, Mpo⁺, Cd117 low	Splenomegaly (spleen = 0.88 g). Leukemic infiltrates in liver and lung. Secondary transplants developed tumors with the same immunophenotype at 24 d.
652	Il7r-IL241-242TC-GFP	78	64.78	Gr1⁺Mac1⁺GFP⁺	IC-Cd3⁺, Cd117 low	Splenomegaly (spleen = 0.73 g). Leukemic infiltrates in brain, eye, kidney, liver, and lung.
653	Il7r-IL241-242TC-GFP	74	33.72	Gr1⁺Mac1⁺GFP⁺	GFP⁺, IC-Cd3⁺, Mpo⁺	Splenomegaly (spleen = 1.25 g). Leukemic infiltrates in brain, kidney, liver, and lung.
655	Il7r-GcinsL243-GFP	71	30.76	Sca1⁺Cd71⁺GFP⁺	GFP⁺, IC-Cd3⁺, Gata1⁺, Runx1⁺, Ly6b⁺, TdT⁺ Cd34⁺	Splenomegaly (spleen = 0.96 g). Leukemic infiltrates in liver, lung, focally in brain/meninges.
656^c	Il7r-GcinsL243-GFP	78	32	Cd117⁺Cd71⁺GFP⁺	Pop1: IC-Cd3⁺, Runx1⁺, Pop2: IC-Cd3⁺, Runx1⁺ and Gata-1⁺	Splenomegaly (spleen = 0.77 g). Leukemic infiltrates in liver and lung.
657	Il7r-GcinsL243-GFP	43	49.46	Sca1⁺Cd71⁺GFP⁺	IC-Cd3⁺, Gata1⁺, Runx1⁺, Ly6b⁺, TdT⁺, Cd117⁺, Fe/80⁺, Mac2⁺	Splenomegaly (spleen = 0.88 g). Leukemic infiltrates in liver, lung, focally in brain/meninges.
658^c	Il7r-GcinsL243-GFP	96	99.9	Cd71⁺GFP⁺	Pop1: IC-Cd3⁺, Runx1⁺, Pop2: IC-Cd3+, Runx1⁺ and Gata-1⁺	Splenomegaly (spleen = 0.9 g). Leukemic infiltrates in liver and lung. Secondary transplants developed identical tumors at 24 d.
659	Il7r-GcinsL243-GFP	71	48.89	Cd4⁺Cd8⁺GFP⁺	IC-Cd3⁺Tdt⁺	The only T-ALL case. Splenomegaly (spleen = 0.54 g). Enlarged thymus (thymus = 0.132 g). Leukemic infiltrates in liver, lung, brain, and kidney.
242^d	Lmo2-mCherry	89	44.3	mCherry⁺Gr1⁺Mac1⁺	IC-Cd3⁺Mpo⁺	All animals had splenomegaly (range: spleen = 0.258–1.163 g). Enlarged thymus (range: thymus = 0.064–0.116 g). Leukemic infiltrates in lungs, liver, lymph nodes, and meninges.
243^d, 244^d, 245^d	Lmo2-mCherry	71	195-528	mCherry⁺Gr1⁺Mac1⁺
611	Lmo2-mCherry	154	36.18	Cd117⁺, Cd71⁺, B220⁺, mCherry⁺	Cd117 weakly +	Splenomegaly (spleen = 0.385 g). Leukemic infiltrates in lung, spleen, liver, BM, and meninges.
612	Lmo2-mCherry	111	20.42	Gr1⁺, Mac1⁺, Cd117⁺, Cd71⁺, Cd5^low, mCherry⁺	Cd117⁺, Cd43⁺	Splenomegaly (spleen = 0.825 g). Leukemic infiltrates in liver, spleen, and kidney.

IC-Cd3⁻ Intracytoplasmic Cd3, Mpo-myeloperoxidase, TdT-terminal deoxynucleotidyl transferase.

Where present, the spleen, thymus, BM, and peripheral blood were immunophenotyped. All tissues were tested for Cd4, Cd8, B220, Cd3, and Cd25. All tumor samples were positive for Cd44.

In the majority of cases where IHC was performed, the samples were tested for Mpo, cKit/Cd117, Cd43, RUNX1, Cd3, Cd5, TdT, Gata-1, B220, Pax5, and Cd34. The markers that had positive reactivity are indicated in the text

These animals contained two distinct populations in their spleen, which could be distinguished by immunohistochemistry as indicated in the text.

Secondary transplant Rag2^−/−, γ_C^−/− recipients that received vector-positive thymocytes from a WT C57BL/6J animal that had been transplanted with 2 × 10⁵ Lmo2, Arf^−/−, and DN2 thymocytes 21 wk before sacrifice.

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