Role of CDK-mediated phosphorylation of CtIP at T847 on embryonic viability
| CtIPT847ACtIP+/− x CtIP+/− | ||||||
| Genotype | CtIP−/− | CtIP−/− | CtIP+/+ | CtIPT847A CtIP−/− | CtIPT847A CtIP+/− | CtIPT847A CtIP+/+ |
| E9.5 embryos | ||||||
| Observed | 0 | 2 | 1 | 0 | 14 | 6 |
| Expected | 2.875 | 5.75 | 2.875 | 2.875 | 5.75 | 2.875 |
| Total embryos screened | 23 | |||||
| CtIPT847ACtIP+/− x CtIP+/− | ||||||
| Genotype | CtIP−/− | CtIP−/− | CtIP+/+ | CtIPT847A CtIP−/− | CtIPT847A CtIP+/− | CtIPT847A CtIP+/+ |
| E9.5 embryos | ||||||
| Observed | 0 | 2 | 1 | 0 | 14 | 6 |
| Expected | 2.875 | 5.75 | 2.875 | 2.875 | 5.75 | 2.875 |
| Total embryos screened | 23 | |||||
CtIP heterozygous mice were intercrossed with one parent carrying a copy of the transgenic CtIP-T847A mutation, and embryos were genotyped at E9.5.