Summary of somatic mutations observed in the Vh186.2 sequences from GC B cells
| Chimera type | n | FWR (1+2+3) | CDR (1+2) | ||
| # R | # S | #R | #S | ||
| WT + TCR-β−/− | 54 | 2.69 ± 1.78 | 0.98 ± 1.04 | 2.31 ± 1.24 | 0.31 ± 0.46 |
| BLyS−/− + TCR-β−/− | 69 | 3.17 ± 1.57 | 1.22 ± 1.16 | 1.81 ± 1.06 | 0.51 ± 0.63 |
| Chimera type | n | FWR (1+2+3) | CDR (1+2) | ||
| # R | # S | #R | #S | ||
| WT + TCR-β−/− | 54 | 2.69 ± 1.78 | 0.98 ± 1.04 | 2.31 ± 1.24 | 0.31 ± 0.46 |
| BLyS−/− + TCR-β−/− | 69 | 3.17 ± 1.57 | 1.22 ± 1.16 | 1.81 ± 1.06 | 0.51 ± 0.63 |
GC B cells (Dump−B220+PNA+FashiNP+) were FACS sorted at day 15 after immunization with NP-CGG. Sequences were pooled from two independent experiments. In each experiment, we sorted 2,000 NP+ GC B cells from splenocytes pooled from three WT and three BLyS−/− T cell chimeras. R denotes replacement and S denotes silent mutations. FWR and CDR values represent mean ± SD.