Transplantation of individually sorted Lineageint c-kit+ sca-1+ CD34− cells
| WT;GFP+ | V617F;GFP+ | ||||
| Exp. 1 | n.a. | n.a. | 18/40 (45%) | 2/40 (5.0%) | 1/2 (50%) |
| Exp. 2 | n.a. | n.a. | 5/58 (8.6%) | 2/58 (3.4%) | 1/2 (50%) |
| Total | n.a. | n.a. | 23/98 (23.5%) | 4/98 (4.1%) | 2/4 (50%) |
| Calculated frequencies | |||||
| ST-HSC | LT-HSC | ST-HSC | LT-HSC | MPN initiating cells | |
| n.a. | n.a. | 1 in 4.3 | 1 in 24.4 | 1 in 2 | |
| WT;GFP+ | V617F;GFP+ | ||||
| Exp. 1 | n.a. | n.a. | 18/40 (45%) | 2/40 (5.0%) | 1/2 (50%) |
| Exp. 2 | n.a. | n.a. | 5/58 (8.6%) | 2/58 (3.4%) | 1/2 (50%) |
| Total | n.a. | n.a. | 23/98 (23.5%) | 4/98 (4.1%) | 2/4 (50%) |
| Calculated frequencies | |||||
| ST-HSC | LT-HSC | ST-HSC | LT-HSC | MPN initiating cells | |
| n.a. | n.a. | 1 in 4.3 | 1 in 24.4 | 1 in 2 | |
Single sorted GFP-positive HSCs were injected into lethally irradiated recipients together with 200,000 GFP-negative WT BM cells. Reconstitution was scored based on the presence of >1% GFP-positive cells in the PB Gr1+ cells at ∼4 mo after transplantation. n.a., not applicable.