Summary of transplantation of individually sorted Lineage− c-kit+ sca-1+ CD34− CD48− Flk2− CD150+ cells
| Experiment number | WT;GFP+ | V617F;GFP+ | |||
| Number and % of mice with short-term reconstitution | Number and % of mice with long-term reconstitution | Number and % of mice with short-term reconstitution | Number and % of mice with long-term reconstitution | Number and % of mice with MPN | |
| Exp. 1 | 6/15 (40.0%) | 2/15 (13.3%) | 6/17 (35.2%) | 2/17 (11.8%) | 0/2 (0%) |
| Exp. 2 | 4/16 (25.0%) | 1/16 (6.3%) | 6/27 (22.2%) | 2/27 (7.4%) | 1/2 (50%) |
| Exp. 3 | 7/11 (63.6%) | 2/11 (18.2%) | 13/38 (34.2%) | 1/38 (2.6%) | 0/1 (0%) |
| Exp. 4 | 0/6 (0%) | 0/6 (0%) | 3/31 (9.7%) | 1/31 (3.2%) | 0/1 (0%) |
| Total | 17/48 (35.4%) | 5/48 (10.4%) | 28/113 (24.8%) | 6/113 (5.3%) | 1/6 (16.7%) |
| Calculated frequencies | |||||
| ST-HSC | LT-HSC | ST-HSC | LT-HSC | MPN initiating cells | |
| 1 in 2.8 | 1 in 9.6 | 1 in 4.5 | 1 in 18.8 | 1 in 6 | |
| Experiment number | WT;GFP+ | V617F;GFP+ | |||
| Number and % of mice with short-term reconstitution | Number and % of mice with long-term reconstitution | Number and % of mice with short-term reconstitution | Number and % of mice with long-term reconstitution | Number and % of mice with MPN | |
| Exp. 1 | 6/15 (40.0%) | 2/15 (13.3%) | 6/17 (35.2%) | 2/17 (11.8%) | 0/2 (0%) |
| Exp. 2 | 4/16 (25.0%) | 1/16 (6.3%) | 6/27 (22.2%) | 2/27 (7.4%) | 1/2 (50%) |
| Exp. 3 | 7/11 (63.6%) | 2/11 (18.2%) | 13/38 (34.2%) | 1/38 (2.6%) | 0/1 (0%) |
| Exp. 4 | 0/6 (0%) | 0/6 (0%) | 3/31 (9.7%) | 1/31 (3.2%) | 0/1 (0%) |
| Total | 17/48 (35.4%) | 5/48 (10.4%) | 28/113 (24.8%) | 6/113 (5.3%) | 1/6 (16.7%) |
| Calculated frequencies | |||||
| ST-HSC | LT-HSC | ST-HSC | LT-HSC | MPN initiating cells | |
| 1 in 2.8 | 1 in 9.6 | 1 in 4.5 | 1 in 18.8 | 1 in 6 | |
Single-sorted GFP-positive HSCs were injected into lethally irradiated recipients together with 200,000 GFP-negative WT BM cells. Reconstitution was scored based on the presence of >1% GFP-positive cells in the PB Gr1+ cells at ∼4 mo after transplantation. n.a., not applicable.