Table 1.

Normal lymphoid and myeloid development in PU.1155−/155− mice

  No. of cells Pax-5 (MFI) 
 WT PU.1155−/155− miR-155−/− WT PU.1155−/155− miR-155−/− 
Marrow       
Pre-proa 2 × 105 ± 1 × 105 3.0 × 106 ± 5 × 104 2.6 × 105 ± 8 × 104    
Pre Bb 1.8 × 106 ± 1 × 105 2.9 × 106 ± 7 × 105 2.2 × 106 ± 6 × 105 2.2 × 104 ± 3 × 103 2.4 × 104 ± 3 × 103 2.3 × 104 ± 3 × 103 
Immature Bc 9 × 105 ± 1 × 105 1.2 × 106 ± 3 × 105 9 × 105 ± 2 × 105 2.8 × 104 ± 4 × 103 3.0 × 104 ± 3 × 103 3.0 × 104 ± 3 × 103 
Mature Bd 9 × 105 ± 2 × 105 1.2 × 106 ± 3 × 105 1.1 × 106 ± 1 × 105 1.2 × 104 ± 1 × 103 1.3 × 104 ± 6 × 102 1.3 × 104 ± 1 × 103 
Myeloide 2.2 × 107 ± 5 × 106 2.3 × 107 ± 2 × 106 1.9 × 107 ± 3 × 106 2.2 × 104 ± 3 × 103 2.4 × 104 ± 3 × 103 2.3 × 104 ± 3 × 103 
Spleen    +++   
CD4 T 2.9 × 106 ± 6 × 105 2.5 × 106 ± 7 × 105 2.7 × 106 ± 9 × 105    
CD8 T 2.2 × 106 ± 9 × 105 2.5 × 106 ± 1 × 105 2.5 × 106 ± 8 × 105    
T1 B cellsf 5.1 × 105 ± 2 × 105 4.8 × 105 ± 2 × 105 8.3 × 105 ± 2 × 105 1.7 × 104 ± 2 × 102 1.6 × 104 ± 8 × 102 1.6 × 104 ± 7 × 102 
T2 B cellsg 4.9 × 105 ± 2 × 105 5.3 × 105 ± 3 × 105 7.0 × 105 ± 1 × 105 1.7 × 104 ± 3 × 102 1.5 × 104 ± 6 × 102 1.5 × 104 ± 8 × 102 
T3 B cellsh 2.7 × 105 ± 9 × 104 2.7 × 105 ± 1 × 105 3.4 × 105 ± 6 × 104 1.4 × 104 ± 1 × 103 1.3 × 104 ± 5 × 102 1.3 × 104 ± 6 × 102 
Fo B cellsi 1.6 × 107 ± 5 × 106 1.6 × 107 ± 5 × 106 1.7 × 107 ± 2 × 106 9.5 × 103 ± 2 × 102 8.8 × 104 ± 4 × 102 8.6 × 103 ± 4 × 102 
MZ B cellsj 8.7 × 105 ± 2 × 105 7.4 × 105 ± 2 × 105 1.4 × 106 ± 3 × 105 1.4 × 104 ± 2 × 102 1.2 × 104 ± 6 × 102 1.2 × 104 ± 7 × 102 
Myeloid cellsk 4 × 106 ± 1 × 106 4 × 106 ± 1 × 106 5 × 106 ± 1 × 106    
Peritoneal cavity       
B1al B1a i 1.3 × 105 ± 5 × 104 2 × 105 ± 1 × 105 9.0 × 103 ± 9 × 102 8.4 × 103 ± 6 × 102 7.5 × 103 ± 7 × 102 
B1bm B1b ii 7 × 104 ± 3 × 104 9 × 104 ± 5 × 104 8.2 × 103 ± 4 × 102 8.1 × 103 ± 5 × 102 7.5 × 103 ± 5 × 102 
B2n B2 iii 1.6 × 105 ± 7 × 104 5 × 105 ± 3 × 105 8.4 × 103 ± 8 × 102 8.5 × 103 ± 7 × 102 7.7 × 103 ± 8 × 102 
Myeloido Myeloid iv 7 × 105 ± 4 × 105 1.2 × 106 ± 8 × 105    
  No. of cells Pax-5 (MFI) 
 WT PU.1155−/155− miR-155−/− WT PU.1155−/155− miR-155−/− 
Marrow       
Pre-proa 2 × 105 ± 1 × 105 3.0 × 106 ± 5 × 104 2.6 × 105 ± 8 × 104    
Pre Bb 1.8 × 106 ± 1 × 105 2.9 × 106 ± 7 × 105 2.2 × 106 ± 6 × 105 2.2 × 104 ± 3 × 103 2.4 × 104 ± 3 × 103 2.3 × 104 ± 3 × 103 
Immature Bc 9 × 105 ± 1 × 105 1.2 × 106 ± 3 × 105 9 × 105 ± 2 × 105 2.8 × 104 ± 4 × 103 3.0 × 104 ± 3 × 103 3.0 × 104 ± 3 × 103 
Mature Bd 9 × 105 ± 2 × 105 1.2 × 106 ± 3 × 105 1.1 × 106 ± 1 × 105 1.2 × 104 ± 1 × 103 1.3 × 104 ± 6 × 102 1.3 × 104 ± 1 × 103 
Myeloide 2.2 × 107 ± 5 × 106 2.3 × 107 ± 2 × 106 1.9 × 107 ± 3 × 106 2.2 × 104 ± 3 × 103 2.4 × 104 ± 3 × 103 2.3 × 104 ± 3 × 103 
Spleen    +++   
CD4 T 2.9 × 106 ± 6 × 105 2.5 × 106 ± 7 × 105 2.7 × 106 ± 9 × 105    
CD8 T 2.2 × 106 ± 9 × 105 2.5 × 106 ± 1 × 105 2.5 × 106 ± 8 × 105    
T1 B cellsf 5.1 × 105 ± 2 × 105 4.8 × 105 ± 2 × 105 8.3 × 105 ± 2 × 105 1.7 × 104 ± 2 × 102 1.6 × 104 ± 8 × 102 1.6 × 104 ± 7 × 102 
T2 B cellsg 4.9 × 105 ± 2 × 105 5.3 × 105 ± 3 × 105 7.0 × 105 ± 1 × 105 1.7 × 104 ± 3 × 102 1.5 × 104 ± 6 × 102 1.5 × 104 ± 8 × 102 
T3 B cellsh 2.7 × 105 ± 9 × 104 2.7 × 105 ± 1 × 105 3.4 × 105 ± 6 × 104 1.4 × 104 ± 1 × 103 1.3 × 104 ± 5 × 102 1.3 × 104 ± 6 × 102 
Fo B cellsi 1.6 × 107 ± 5 × 106 1.6 × 107 ± 5 × 106 1.7 × 107 ± 2 × 106 9.5 × 103 ± 2 × 102 8.8 × 104 ± 4 × 102 8.6 × 103 ± 4 × 102 
MZ B cellsj 8.7 × 105 ± 2 × 105 7.4 × 105 ± 2 × 105 1.4 × 106 ± 3 × 105 1.4 × 104 ± 2 × 102 1.2 × 104 ± 6 × 102 1.2 × 104 ± 7 × 102 
Myeloid cellsk 4 × 106 ± 1 × 106 4 × 106 ± 1 × 106 5 × 106 ± 1 × 106    
Peritoneal cavity       
B1al B1a i 1.3 × 105 ± 5 × 104 2 × 105 ± 1 × 105 9.0 × 103 ± 9 × 102 8.4 × 103 ± 6 × 102 7.5 × 103 ± 7 × 102 
B1bm B1b ii 7 × 104 ± 3 × 104 9 × 104 ± 5 × 104 8.2 × 103 ± 4 × 102 8.1 × 103 ± 5 × 102 7.5 × 103 ± 5 × 102 
B2n B2 iii 1.6 × 105 ± 7 × 104 5 × 105 ± 3 × 105 8.4 × 103 ± 8 × 102 8.5 × 103 ± 7 × 102 7.7 × 103 ± 8 × 102 
Myeloido Myeloid iv 7 × 105 ± 4 × 105 1.2 × 106 ± 8 × 105    

Data corresponds to mean and standard deviation from five mice each genotype. Data analysis in is based on one experiment with 4-5 biological replicates per genotype.

a

B220+/low, IgM- cKit+

b

B220low, IgM-, CD25+

c

B220low, IgM+

d

B220+/IgM+

e

Gr-1+/Mac-1+

f

B220low,CD93+, CD23low, IgMhigh

g

B220low,CD93+, CD23high, IgMhigh

h

B220low,CD93+, CD23high, IgMlow.

i

B220high,CD93-, CD23+, IgM+

j

B220high,CD93-, CD23-, IgMhigh, CD21high

k

Gr-1+/Mac-1+

l

CD5+,Mac-1+,B220low

m

CD5-,Mac-1+,B220low

n

CD43low,Mac-1-,B220low

o

Gr-1+/Mac-1+

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