CMS5 and Meth A epitopes with highest DAI scores
| Gene | Mut/WT sequence | Mut/WT Score | DAI | Measured IC50 for Kd (Mut/WT) | ICS | ELISpot | Tumor rejection |
| CMS5 Epitopes | |||||||
| Dhx8.1 | P(y/D)LTQYAIIML | 9.3/-2.3 | 11.6 | 2192/1653 | - | +++++ | - |
| Alkbh6.1 | D(y/D)VPMEQP | 4.3/-7.3 | 11.6 | 60858/− | - | - | - |
| Dhx8.2 | P(y/D)LTQYAI | 9.9/-1.6 | 11.5 | −a/− | - | +++++ | - |
| Dhx8.3 | P(y/D)LTQYAII | 9.3/-2.2 | 11.5 | 244/1418 | + | +++++ | - |
| Alkbh6.2 | D(y/D)VPMEQPR | 6.8/-4.7 | 11.5 | −/51229 | - | ++ | + |
| Dhx8.4 | P(y/D)LTQYAIIM | 6.6/-4.9 | 11.5 | 6571/6256 | - | +++ | - |
| Alkbh6.3 | D(y/D)VPMEQPRP | 4.8/-6.7 | 11.5 | −/23570 | - | - | - |
| Alkbh6.4 | D(y/D)VPMEQPRPP | 4.7/-6.8 | 11.5 | 47053/2957 | - | - | - |
| Rangap1 | SEDKIKAI(l/P) | 1.4/-5.4 | 6.8 | −/5108 | - | +++++ | - |
| Stau1.1 | LKSEEKT(l/P) | 0.6/-6.2 | 6.8 | −/− | - | - | - |
| Stau1.2 | KPALKSEEKT(l/P) | 0.1/-6.7 | 6.8 | −/− | - | - | - |
| Stau1.3 | PALKSEEKT(l/P) | 1.3/-5.4 | 6.7 | 69546/− | - | - | - |
| Stau1.4 | ALKSEEKT(l/P) | 1.2/-5.5 | 6.7 | −/− | - | - | + |
| 9430016H08Rik | SWSSRRSLLG(l/R) | 5.9/-0.6 | 6.5 | −/− | - | +++++ | - |
| Slit3 | GFHGCIHEV(l/R) | 4.7/-1.8 | 6.5 | 51640/− | + | +++ | + |
| Atxn10.1 | QVFPGLME(l/R) | 3.4/-3.1 | 6.5 | −/7054 | + | +++ | + |
| Sipa1l3 | TTTPGGRPPY(l/R) | 2.7/-3.8 | 6.5 | −/− | - | ++++ | - |
| Atxn10.2 | VFPGLME(l/R) | 2.5/-4 | 6.5 | −/1107 | - | - | + |
| Ccdc136 | ELQGLLEDE(l/R) | 2.4/-4.1 | 6.5 | −/4537 | - | - | + |
| Mast2 | KLQRQYRSPR(l/R) | 2.2/-4.3 | 6.5 | 10107/8511 | - | - | - |
| Meth A Epitopes | |||||||
| Tnpo3.1 | (sy/LD)MLQALCI | 8.2/-5.2 | 13.4 | 82/146 | + | +++++ | + |
| Tnpo3.2 | (sy/LD)MLQALCIPT | 7.1/-6.3 | 13.4 | 9964/85 | + | +++ | - |
| Tnpo3.3 | (sy/LD)MLQALCIP | 3/-10.4 | 13.4 | 67/111 | + | +++++ | - |
| Tnpo3.4 | (sy/LD)MLQALC | 5.9/-7.4 | 13.3 | 14927/89 | - | - | - |
| Trim26.1 | A(y/D)ILAALTKL | 12.8/1.2 | 11.6 | 622/1.1 | - | +++++ | - |
| Nus1 | P(y/D)LVLKFGPV | 10.5/-1.1 | 11.6 | 2359/1.9 | - | - | - |
| Tpst2.1 | L(y/D)EAGVTDEV | 10.3/-1.3 | 11.6 | 60/− | - | +++ | - |
| Fiz1 | H(y/D)LQGSNA | 10.3/-1.3 | 11.6 | 2473/− | - | - | - |
| Kdm4b | L(y/D)HTRPTAL | 10/-1.6 | 11.6 | 264/− | - | - | - |
| Dis3l2.1 | I(y/D)GVVARNRAL | 9.3/-2.3 | 11.6 | 143/− | + | + | - |
| Ube4a.1 | A(y/D)AKQFAAI | 9.3/-2.3 | 11.6 | 12/− | + | + | - |
| Ncdn | S(y/D)CEPALNQA | 8.9/-2.7 | 11.6 | 664/− | + | - | - |
| Gapdh | V(y/D)LTCRLEKPA | 8.9/-2.7 | 11.6 | 1150/− | - | - | - |
| Ckap5 | K(y/D)MSMLEER | 8.1/-3.5 | 11.6 | 58/− | - | +++ | - |
| Prrc2a | P(y/D)KRLKAEPA | 7.9/-3.7 | 11.6 | 1450/261 | - | - | - |
| Tmx3 | D(y/D)IIEFAHRV | 7.3/-4.3 | 11.6 | 7941/351 | - | - | - |
| Nfkb1 | G(y/D)SVLHLAI | 6.9/-4.6 | 11.5 | 0.26/1615 | + | - | + |
| Dis3l2.2 | I(y/D)GVVARNRA | 6.9/-4.7 | 11.6 | 1342/262 | - | - | - |
| Ugdh | L(y/D)YERIHKKML | 6.4/-5.2 | 11.6 | 600/12 | - | - | - |
| Mll2 | S(y/D)RLPSSRKK | 5.9/-5.7 | 11.6 | 6673/17 | - | + | - |
| Galnt1 | L(y/D)VSKLNGP | 5.6/-6 | 11.6 | 27086/− | - | - | - |
| Tpst2.2 | L(y/D)EAGVTDE | 5.5/-6.1 | 11.6 | −/− | - | +++ | - |
| Cpsf2 | L(y/D)DVDAAF | 5.3/-6.3 | 11.6 | 1410/− | - | +++ | + |
| Zfp236.1 | E(y/D)LDLQTQ | 5.3/-6.3 | 11.6 | 1641/− | - | +++ | + |
| Trim26.2 | A(y/D)ILAALTKLQ | 4.9/-6.7 | 11.6 | 17599/− | - | - | - |
| Zfp236.2 | E(y/D)LDLQTQG | 4.9/-6.7 | 11.6 | 10028/− | - | ++++ | - |
| Ube4a.2 | A(y/D)AKQFAA | 4.7/-6.9 | 11.6 | 28583/− | - | - | - |
| Dcaf6 | A(y/D)RLEGDRS | 3.7/-7.9 | 11.6 | −/− | - | - | - |
| Gene | Mut/WT sequence | Mut/WT Score | DAI | Measured IC50 for Kd (Mut/WT) | ICS | ELISpot | Tumor rejection |
| CMS5 Epitopes | |||||||
| Dhx8.1 | P(y/D)LTQYAIIML | 9.3/-2.3 | 11.6 | 2192/1653 | - | +++++ | - |
| Alkbh6.1 | D(y/D)VPMEQP | 4.3/-7.3 | 11.6 | 60858/− | - | - | - |
| Dhx8.2 | P(y/D)LTQYAI | 9.9/-1.6 | 11.5 | −a/− | - | +++++ | - |
| Dhx8.3 | P(y/D)LTQYAII | 9.3/-2.2 | 11.5 | 244/1418 | + | +++++ | - |
| Alkbh6.2 | D(y/D)VPMEQPR | 6.8/-4.7 | 11.5 | −/51229 | - | ++ | + |
| Dhx8.4 | P(y/D)LTQYAIIM | 6.6/-4.9 | 11.5 | 6571/6256 | - | +++ | - |
| Alkbh6.3 | D(y/D)VPMEQPRP | 4.8/-6.7 | 11.5 | −/23570 | - | - | - |
| Alkbh6.4 | D(y/D)VPMEQPRPP | 4.7/-6.8 | 11.5 | 47053/2957 | - | - | - |
| Rangap1 | SEDKIKAI(l/P) | 1.4/-5.4 | 6.8 | −/5108 | - | +++++ | - |
| Stau1.1 | LKSEEKT(l/P) | 0.6/-6.2 | 6.8 | −/− | - | - | - |
| Stau1.2 | KPALKSEEKT(l/P) | 0.1/-6.7 | 6.8 | −/− | - | - | - |
| Stau1.3 | PALKSEEKT(l/P) | 1.3/-5.4 | 6.7 | 69546/− | - | - | - |
| Stau1.4 | ALKSEEKT(l/P) | 1.2/-5.5 | 6.7 | −/− | - | - | + |
| 9430016H08Rik | SWSSRRSLLG(l/R) | 5.9/-0.6 | 6.5 | −/− | - | +++++ | - |
| Slit3 | GFHGCIHEV(l/R) | 4.7/-1.8 | 6.5 | 51640/− | + | +++ | + |
| Atxn10.1 | QVFPGLME(l/R) | 3.4/-3.1 | 6.5 | −/7054 | + | +++ | + |
| Sipa1l3 | TTTPGGRPPY(l/R) | 2.7/-3.8 | 6.5 | −/− | - | ++++ | - |
| Atxn10.2 | VFPGLME(l/R) | 2.5/-4 | 6.5 | −/1107 | - | - | + |
| Ccdc136 | ELQGLLEDE(l/R) | 2.4/-4.1 | 6.5 | −/4537 | - | - | + |
| Mast2 | KLQRQYRSPR(l/R) | 2.2/-4.3 | 6.5 | 10107/8511 | - | - | - |
| Meth A Epitopes | |||||||
| Tnpo3.1 | (sy/LD)MLQALCI | 8.2/-5.2 | 13.4 | 82/146 | + | +++++ | + |
| Tnpo3.2 | (sy/LD)MLQALCIPT | 7.1/-6.3 | 13.4 | 9964/85 | + | +++ | - |
| Tnpo3.3 | (sy/LD)MLQALCIP | 3/-10.4 | 13.4 | 67/111 | + | +++++ | - |
| Tnpo3.4 | (sy/LD)MLQALC | 5.9/-7.4 | 13.3 | 14927/89 | - | - | - |
| Trim26.1 | A(y/D)ILAALTKL | 12.8/1.2 | 11.6 | 622/1.1 | - | +++++ | - |
| Nus1 | P(y/D)LVLKFGPV | 10.5/-1.1 | 11.6 | 2359/1.9 | - | - | - |
| Tpst2.1 | L(y/D)EAGVTDEV | 10.3/-1.3 | 11.6 | 60/− | - | +++ | - |
| Fiz1 | H(y/D)LQGSNA | 10.3/-1.3 | 11.6 | 2473/− | - | - | - |
| Kdm4b | L(y/D)HTRPTAL | 10/-1.6 | 11.6 | 264/− | - | - | - |
| Dis3l2.1 | I(y/D)GVVARNRAL | 9.3/-2.3 | 11.6 | 143/− | + | + | - |
| Ube4a.1 | A(y/D)AKQFAAI | 9.3/-2.3 | 11.6 | 12/− | + | + | - |
| Ncdn | S(y/D)CEPALNQA | 8.9/-2.7 | 11.6 | 664/− | + | - | - |
| Gapdh | V(y/D)LTCRLEKPA | 8.9/-2.7 | 11.6 | 1150/− | - | - | - |
| Ckap5 | K(y/D)MSMLEER | 8.1/-3.5 | 11.6 | 58/− | - | +++ | - |
| Prrc2a | P(y/D)KRLKAEPA | 7.9/-3.7 | 11.6 | 1450/261 | - | - | - |
| Tmx3 | D(y/D)IIEFAHRV | 7.3/-4.3 | 11.6 | 7941/351 | - | - | - |
| Nfkb1 | G(y/D)SVLHLAI | 6.9/-4.6 | 11.5 | 0.26/1615 | + | - | + |
| Dis3l2.2 | I(y/D)GVVARNRA | 6.9/-4.7 | 11.6 | 1342/262 | - | - | - |
| Ugdh | L(y/D)YERIHKKML | 6.4/-5.2 | 11.6 | 600/12 | - | - | - |
| Mll2 | S(y/D)RLPSSRKK | 5.9/-5.7 | 11.6 | 6673/17 | - | + | - |
| Galnt1 | L(y/D)VSKLNGP | 5.6/-6 | 11.6 | 27086/− | - | - | - |
| Tpst2.2 | L(y/D)EAGVTDE | 5.5/-6.1 | 11.6 | −/− | - | +++ | - |
| Cpsf2 | L(y/D)DVDAAF | 5.3/-6.3 | 11.6 | 1410/− | - | +++ | + |
| Zfp236.1 | E(y/D)LDLQTQ | 5.3/-6.3 | 11.6 | 1641/− | - | +++ | + |
| Trim26.2 | A(y/D)ILAALTKLQ | 4.9/-6.7 | 11.6 | 17599/− | - | - | - |
| Zfp236.2 | E(y/D)LDLQTQG | 4.9/-6.7 | 11.6 | 10028/− | - | ++++ | - |
| Ube4a.2 | A(y/D)AKQFAA | 4.7/-6.9 | 11.6 | 28583/− | - | - | - |
| Dcaf6 | A(y/D)RLEGDRS | 3.7/-7.9 | 11.6 | −/− | - | - | - |
IC50 > 70,000 nM
Note for ELISpot results: 1–9 spots/106 CD8 = +; 10–20 spots/106 CD8 = ++; 21–50 spots/106 CD8 = +++; 51–100 spots/106 CD8 = ++++; >100 spots/106 CD8 = +++++.