Histomorphometry analysis of Dcamkl1−/− mice
| Parameter | WT (n = 6) | Dcamkl1−/− (n ≥ 5) | P-value |
| BV/TV (%) | 6.13 ± 0.74 | 10.39 ± 1.32a | 0.018 |
| Tb.Th (µm) | 28.22 ± 1.01 | 34.23 ± 1.42b | 0.006 |
| Tb.N (/mm) | 2.18 ± 0.25 | 3.01 ± 0.29 | 0.055 |
| Tb.Sp (µm) | 464 ± 56 | 316 ± 39 | 0.055 |
| MS/BS (%) | 36.68 ± 1.80 | 42.16 ± 1.38a | 0.045 |
| MAR (µm/d) | 1.81 ± 0.05 | 2.16 ± 0.14a | 0.033 |
| BFR/BS (µm3/µm2/yr) | 242 ± 13 | 331 ± 20b | 0.004 |
| BFR/BV (%/yr) | 1,711 ± 36 | 2,016 ± 142a | 0.048 |
| BFR/TV (%/yr) | 105 ± 12 | 184 ± 12b | 0.001 |
| Ob.S/BS (%) | 10.19 ± 2.14 | 15.55 ± 1.27 | 0.057 |
| N.Ob/T.Ar (/mm2) | 33.96 ± 5.19 | 81.52 ± 14.18a | 0.010 |
| N.Ob/BS (/mm) | 8.68 ± 1.62 | 13.26 ± 1.11a | 0.042 |
| OS/BS (%) | 5.56 ± 1.01 | 5.88 ± 0.94 | 0.824 |
| O.Th (µm) | 2.49 ± 0.33 | 2.40 ± 0.29 | 0.854 |
| Oc.S/BS (%) | 0.84 ± 0.21 | 0.57 ± 0.14 | 0.303 |
| N.Oc/T.Ar (/mm2) | 1.74 ± 0.59 | 1.54 ± 0.38 | 0.783 |
| N.Oc/BS (/mm) | 0.37 ± 0.08 | 0.24 ± 0.06 | 0.205 |
| Parameter | WT (n = 6) | Dcamkl1−/− (n ≥ 5) | P-value |
| BV/TV (%) | 6.13 ± 0.74 | 10.39 ± 1.32a | 0.018 |
| Tb.Th (µm) | 28.22 ± 1.01 | 34.23 ± 1.42b | 0.006 |
| Tb.N (/mm) | 2.18 ± 0.25 | 3.01 ± 0.29 | 0.055 |
| Tb.Sp (µm) | 464 ± 56 | 316 ± 39 | 0.055 |
| MS/BS (%) | 36.68 ± 1.80 | 42.16 ± 1.38a | 0.045 |
| MAR (µm/d) | 1.81 ± 0.05 | 2.16 ± 0.14a | 0.033 |
| BFR/BS (µm3/µm2/yr) | 242 ± 13 | 331 ± 20b | 0.004 |
| BFR/BV (%/yr) | 1,711 ± 36 | 2,016 ± 142a | 0.048 |
| BFR/TV (%/yr) | 105 ± 12 | 184 ± 12b | 0.001 |
| Ob.S/BS (%) | 10.19 ± 2.14 | 15.55 ± 1.27 | 0.057 |
| N.Ob/T.Ar (/mm2) | 33.96 ± 5.19 | 81.52 ± 14.18a | 0.010 |
| N.Ob/BS (/mm) | 8.68 ± 1.62 | 13.26 ± 1.11a | 0.042 |
| OS/BS (%) | 5.56 ± 1.01 | 5.88 ± 0.94 | 0.824 |
| O.Th (µm) | 2.49 ± 0.33 | 2.40 ± 0.29 | 0.854 |
| Oc.S/BS (%) | 0.84 ± 0.21 | 0.57 ± 0.14 | 0.303 |
| N.Oc/T.Ar (/mm2) | 1.74 ± 0.59 | 1.54 ± 0.38 | 0.783 |
| N.Oc/BS (/mm) | 0.37 ± 0.08 | 0.24 ± 0.06 | 0.205 |
Tb.Sp, trabecular separation; N.Ob/T.Ar, osteoblast number/tissue area ratio; N.Ob/BS, osteoblast number/bone surface; N.Oc/T.Ar, osteoclast number/tissue area ratio; N.Oc/BS, osteoclast number/bone surface. 9-wk-old male Dcamkl1−/− mice and WT control mice were injected with calcein. 5 d later, mice were injected with calcein again. Mice were sacrificed 3 d after the second injection, and tibias were processed for quantitative histomorphometry. Histomorphometric analysis at proximal tibiae revealed that Dcamkl1−/− mice had significantly higher cancellous bone volume and thicker trabeculae than WT controls.
P < 0.05 compared with WT, unpaired Student’s t test.
P < 0.01 compared with WT, unpaired Student’s t test.