Table 1.

DNA aberrations in in vitro-expanded hematopoietic progenitor cells.

Genotype Break Gap Frag DC CF Trans Radial 30-39 40 41+ Cells Sex 
WT 10 40 50 
WT 43 50 
WT 12 38 50 
SIRT1Δ/Δ 13 37 50 
SIRT1Δ/Δ 10 11 39 50 
SIRT1Δ/Δ 42 50 
Genotype Break Gap Frag DC CF Trans Radial 30-39 40 41+ Cells Sex 
WT 10 40 50 
WT 43 50 
WT 12 38 50 
SIRT1Δ/Δ 13 37 50 
SIRT1Δ/Δ 10 11 39 50 
SIRT1Δ/Δ 42 50 

BM from three Sirt1-E4fl/fl, ERT2-Cre mice (SIRT1Δ/Δ) and three Sirt1-E4fl/fl controls (WT) was cultured in the presence of SCF, FLT3L, IL-6, and TPO, and metaphases were harvested after 10 d. Chromosomal DNA aberrations were scored for 50 metaphases per sample. Frag: DNA fragment; DC: dicentric chromosome; CF: centric fusion; Trans: translocation. Number of chromosomes per cell and gender are indicated.

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