Human peptides screened for the ability to stimulate the AV22 and AV9 Be-specific T cell hybridomas
| ID/rank | Ratio to max | Peptide sequence | GenBank accession no. | AV22 | AV9 | Protein | |||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | IL-2 | IL-2 | ||||
| pg/ml | pg/ml | ||||||||||||||
| HU1 | 0.89 | M | W | A | D | L | L | E | L | I | D | BAD92652.1 | 997 | 1,117 | SPTB |
| HU2 | 0.88 | C | F | L | D | L | L | E | L | L | I | CAE89418.1 | 716 | 966 | unnamed |
| HU3 | 0.87 | A | W | A | D | L | L | E | L | I | D | BAD92985.1 | 29.3 | 75.1 | SPTBN1 |
| HU4 | 0.87 | A | W | A | D | L | L | E | L | L | D | BAA32700.2 | 4.9 | 9.4 | |
| HU5 | 0.87 | C | W | L | D | A | L | E | L | A | L | BAA96058.1 | 4.1 | 4.6 | |
| HU6 | 0.86 | L | D | V | D | L | L | E | L | V | I | BAA23700.1 | 4.9 | 4.1 | |
| HU7 | 0.86 | Y | W | L | D | L | W | L | F | I | L | CAF00026.1 | 4.1 | 3.6 | |
| HU8 | 0.85 | C | W | L | S | L | L | E | Y | L | L | AAD52694.1 | 4.6 | 5.4 | |
| HU9 | 0.85 | F | W | L | R | L | L | E | L | T | W | AAH40508.1 | 21.7 | 48.6 | PRRT3 |
| HU10 | 0.85 | C | L | A | D | L | Y | E | T | L | G | BAC02709.1 | 5.1 | 29 | |
| HU11 | 0.85 | W | W | I | D | L | L | R | R | T | G | CAE89469.1 | 3.3 | 5.6 | |
| HU12 | 0.85 | C | W | L | D | L | L | L | A | A | L | CAD33443.1 | 3.8 | 3.6 | |
| HU13 | 0.84 | L | W | I | L | L | L | E | T | S | L | BAB67803.1 | 3.6 | 4.9 | |
| HU14 | 0.84 | L | S | I | D | L | Y | E | L | I | K | AAG17028.1 | 4.4 | 6.1 | |
| HU15 | 0.84 | F | W | I | D | L | N | E | D | I | I | CAD67506.1 | 6.7 | 49.3 | unnamed |
| HU16 | 0.84 | L | W | L | D | L | W | Y | L | M | F | BAC85487.1 | 4.6 | 6.1 | |
| HU17 | 0.83 | C | T | V | D | L | L | E | F | Q | P | BAC85360.1 | 15.6 | 16.3 | |
| HU18 | 0.83 | H | W | L | D | L | F | R | L | L | G | AAB09728.1 | 4.4 | 4.4 | |
| HU19 | 0.83 | C | W | V | D | L | I | I | S | S | S | CAD38236.1 | 4.1 | 4.6 | |
| HU20 | 0.83 | F | W | A | R | L | L | E | R | L | F | CAF04283.1 | 4.6 | 4.6 | |
| HU21 | 0.83 | Y | C | I | D | L | L | E | R | L | A | CAC69378.1 | 17.4 | 48.8 | unnamed |
| HU22 | 0.83 | F | W | A | Q | L | L | E | R | V | F | BAC04745.1 | 5.1 | 4.1 | |
| HU23 | 0.83 | F | W | T | P | L | L | E | S | L | A | AAH81559.1 | 4.4 | 4.4 | |
| HU24 | 0.83 | L | C | L | D | L | L | E | L | S | T | CAI40388.1 | 5.4 | 4.6 | |
| HU25 | 0.82 | F | V | D | D | L | F | E | T | L | L | AAH09343.2 | 622 | 847 | PLXNA2 |
| HU26 | 0.82 | L | N | V | D | L | Y | E | L | S | S | BAA92381.1 | 4.9 | 7.1 | |
| HU28 | 0.82 | L | C | A | D | L | F | E | R | V | P | BAD96476.1 | 4.4 | 4.6 | |
| HU29 | 0.82 | W | W | Q | D | L | F | R | I | V | L | CAE90529.1 | 3.3 | 4.6 | |
| HU30 | 0.81 | L | K | V | D | L | L | E | Q | T | K | CAI22508.1 | 3.1 | 3.9 | |
| HU31 | 0.81 | F | V | D | D | L | F | E | T | I | F | BAB13376.3 | 973 | 1,042 | PLXNA4 |
| HU32 | 0.81 | F | E | A | D | L | L | E | M | A | E | CAF06542.1 | 5.4 | 7.9 | |
| HU33 | 0.81 | F | V | D | D | L | F | E | T | L | F | CAD35002.1 | 850 | 954 | PLXNA2 |
| HU35 | 0.81 | F | V | D | D | L | F | E | T | V | F | CAI43194.1 | 947 | 1,035 | PLXNA3 |
| HU38 | 0.81 | L | K | L | D | L | L | E | A | N | S | BAE46614.1 | 5.6 | 5.1 | |
| HU39 | 0.81 | F | W | L | P | L | L | E | K | V | Y | CAF85973.1 | 22.4 | 68.7 | unnamed |
| ID/rank | Ratio to max | Peptide sequence | GenBank accession no. | AV22 | AV9 | Protein | |||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | IL-2 | IL-2 | ||||
| pg/ml | pg/ml | ||||||||||||||
| HU1 | 0.89 | M | W | A | D | L | L | E | L | I | D | BAD92652.1 | 997 | 1,117 | SPTB |
| HU2 | 0.88 | C | F | L | D | L | L | E | L | L | I | CAE89418.1 | 716 | 966 | unnamed |
| HU3 | 0.87 | A | W | A | D | L | L | E | L | I | D | BAD92985.1 | 29.3 | 75.1 | SPTBN1 |
| HU4 | 0.87 | A | W | A | D | L | L | E | L | L | D | BAA32700.2 | 4.9 | 9.4 | |
| HU5 | 0.87 | C | W | L | D | A | L | E | L | A | L | BAA96058.1 | 4.1 | 4.6 | |
| HU6 | 0.86 | L | D | V | D | L | L | E | L | V | I | BAA23700.1 | 4.9 | 4.1 | |
| HU7 | 0.86 | Y | W | L | D | L | W | L | F | I | L | CAF00026.1 | 4.1 | 3.6 | |
| HU8 | 0.85 | C | W | L | S | L | L | E | Y | L | L | AAD52694.1 | 4.6 | 5.4 | |
| HU9 | 0.85 | F | W | L | R | L | L | E | L | T | W | AAH40508.1 | 21.7 | 48.6 | PRRT3 |
| HU10 | 0.85 | C | L | A | D | L | Y | E | T | L | G | BAC02709.1 | 5.1 | 29 | |
| HU11 | 0.85 | W | W | I | D | L | L | R | R | T | G | CAE89469.1 | 3.3 | 5.6 | |
| HU12 | 0.85 | C | W | L | D | L | L | L | A | A | L | CAD33443.1 | 3.8 | 3.6 | |
| HU13 | 0.84 | L | W | I | L | L | L | E | T | S | L | BAB67803.1 | 3.6 | 4.9 | |
| HU14 | 0.84 | L | S | I | D | L | Y | E | L | I | K | AAG17028.1 | 4.4 | 6.1 | |
| HU15 | 0.84 | F | W | I | D | L | N | E | D | I | I | CAD67506.1 | 6.7 | 49.3 | unnamed |
| HU16 | 0.84 | L | W | L | D | L | W | Y | L | M | F | BAC85487.1 | 4.6 | 6.1 | |
| HU17 | 0.83 | C | T | V | D | L | L | E | F | Q | P | BAC85360.1 | 15.6 | 16.3 | |
| HU18 | 0.83 | H | W | L | D | L | F | R | L | L | G | AAB09728.1 | 4.4 | 4.4 | |
| HU19 | 0.83 | C | W | V | D | L | I | I | S | S | S | CAD38236.1 | 4.1 | 4.6 | |
| HU20 | 0.83 | F | W | A | R | L | L | E | R | L | F | CAF04283.1 | 4.6 | 4.6 | |
| HU21 | 0.83 | Y | C | I | D | L | L | E | R | L | A | CAC69378.1 | 17.4 | 48.8 | unnamed |
| HU22 | 0.83 | F | W | A | Q | L | L | E | R | V | F | BAC04745.1 | 5.1 | 4.1 | |
| HU23 | 0.83 | F | W | T | P | L | L | E | S | L | A | AAH81559.1 | 4.4 | 4.4 | |
| HU24 | 0.83 | L | C | L | D | L | L | E | L | S | T | CAI40388.1 | 5.4 | 4.6 | |
| HU25 | 0.82 | F | V | D | D | L | F | E | T | L | L | AAH09343.2 | 622 | 847 | PLXNA2 |
| HU26 | 0.82 | L | N | V | D | L | Y | E | L | S | S | BAA92381.1 | 4.9 | 7.1 | |
| HU28 | 0.82 | L | C | A | D | L | F | E | R | V | P | BAD96476.1 | 4.4 | 4.6 | |
| HU29 | 0.82 | W | W | Q | D | L | F | R | I | V | L | CAE90529.1 | 3.3 | 4.6 | |
| HU30 | 0.81 | L | K | V | D | L | L | E | Q | T | K | CAI22508.1 | 3.1 | 3.9 | |
| HU31 | 0.81 | F | V | D | D | L | F | E | T | I | F | BAB13376.3 | 973 | 1,042 | PLXNA4 |
| HU32 | 0.81 | F | E | A | D | L | L | E | M | A | E | CAF06542.1 | 5.4 | 7.9 | |
| HU33 | 0.81 | F | V | D | D | L | F | E | T | L | F | CAD35002.1 | 850 | 954 | PLXNA2 |
| HU35 | 0.81 | F | V | D | D | L | F | E | T | V | F | CAI43194.1 | 947 | 1,035 | PLXNA3 |
| HU38 | 0.81 | L | K | L | D | L | L | E | A | N | S | BAE46614.1 | 5.6 | 5.1 | |
| HU39 | 0.81 | F | W | L | P | L | L | E | K | V | Y | CAF85973.1 | 22.4 | 68.7 | unnamed |
Designations in bold indicate peptides that stimulate AV22 and AV9 in the presence of BeSO4. For Ratio to max, the score of each peptide was divided by the matrix maximum score. Peptide positions are shown in bold if peptides contain W2D4L5E7. Protein designations (if available) are provided only for peptides that gave a positive response. Hybridomas were stimulated with 0.5 µg/ml of peptide and BeSO4 at 75 µM.