TRAV and TRBV expression on reactive clones
| Clone | PCR | Flow cytometry | ||||
| Vα gene | Vβ gene | Anti-TRAV14 | Anti-TRAV12 | Anti-TRBV31 | Resulting phenotype | |
| 15-2 | TRAV14, 3 and 20 | TRBV31 and 12.1 | negative | – | positive | TRAV3/TRBV31 |
| 45-1 | TRAV4 and 20 | TRBV31 and 12.1 | – | – | positive | TRAV4/TRBV31 |
| 48-5 | TRAV12, 13 and 20 | TRBV31 and 12.1 | – | negative | positive | TRAV13/TRBV31 |
| Clone | PCR | Flow cytometry | ||||
| Vα gene | Vβ gene | Anti-TRAV14 | Anti-TRAV12 | Anti-TRBV31 | Resulting phenotype | |
| 15-2 | TRAV14, 3 and 20 | TRBV31 and 12.1 | negative | – | positive | TRAV3/TRBV31 |
| 45-1 | TRAV4 and 20 | TRBV31 and 12.1 | – | – | positive | TRAV4/TRBV31 |
| 48-5 | TRAV12, 13 and 20 | TRBV31 and 12.1 | – | negative | positive | TRAV13/TRBV31 |
The cDNA from reactive clones was amplified by PCR using appropriate Vβ-specific 5′ primers with a constant region Cβ 3′ primer, or relevant Vα-specific 5′ primers with a constant region Cα 3′ primer (Tables S1 and S2). Further phenotype of Vα and Vβ chain expression was analyzed by flow cytometry using antibodies to TRAV14, TRAV12, and TRBV31.