Proteins copurifying with AID-Flag-HA from Ramos B cells identified by mass spectrometry
| HUGO name | Mascot score | Coverage | Description |
| % | |||
| HSP90AB1 | 2,178 | 44 | Heat shock 90-kD protein 1, β |
| 300 | 8 | ||
| HSP90AA1 | 1,668 | 35 | Heat shock 90-kD protein 1, α |
| 151 | 6 | ||
| HSPA8 | 1,338 | 39 | Heat shock 70-kD protein 8 isoform 1 |
| HSPA6 | 327 | 8 | Heat shock 70-kD protein B |
| AHSA1 | 81 | 3 | AHA-1 activator of heat shock 90-kD protein ATPase homologue 1 |
| 27 | 9 | ||
| DNAJA1 | 212 | 26 | Hsp40 homologue, subfamily A, member 1 |
| PSMD2 | 242 | 14 | Proteasome 26S non-ATPase subunit 2 |
| PSMD1 | 105 | 2 | Proteasome 26S non-ATPase subunit 1 |
| PSMD6 | 105 | 5 | Proteasome 26S non-ATPase subunit 6 |
| 46 | 6 | ||
| PSMC2 | 75 | 3 | Proteasome 26S ATPase subunit 2 |
| HUGO name | Mascot score | Coverage | Description |
| % | |||
| HSP90AB1 | 2,178 | 44 | Heat shock 90-kD protein 1, β |
| 300 | 8 | ||
| HSP90AA1 | 1,668 | 35 | Heat shock 90-kD protein 1, α |
| 151 | 6 | ||
| HSPA8 | 1,338 | 39 | Heat shock 70-kD protein 8 isoform 1 |
| HSPA6 | 327 | 8 | Heat shock 70-kD protein B |
| AHSA1 | 81 | 3 | AHA-1 activator of heat shock 90-kD protein ATPase homologue 1 |
| 27 | 9 | ||
| DNAJA1 | 212 | 26 | Hsp40 homologue, subfamily A, member 1 |
| PSMD2 | 242 | 14 | Proteasome 26S non-ATPase subunit 2 |
| PSMD1 | 105 | 2 | Proteasome 26S non-ATPase subunit 1 |
| PSMD6 | 105 | 5 | Proteasome 26S non-ATPase subunit 6 |
| 46 | 6 | ||
| PSMC2 | 75 | 3 | Proteasome 26S ATPase subunit 2 |
Proteins with two rows of data were identified in two independent experiments. The gel from one of the experiments is shown in Fig. 1 A. A threshold Mascot score of 50 was used as cut-off, indicating a 95% confidence of being a true identification. For AHSA1, in the second experiment, the mass spectrometry profile was manually examined to confirm the reliability of the observation.