Table I. Proteins copurifying with... | Rockefeller University Press

Table I.

Proteins copurifying with AID-Flag-HA from Ramos B cells identified by mass spectrometry

HUGO name	Mascot score	Coverage	Description
		%
HSP90AB1	2,178	44	Heat shock 90-kD protein 1, β
	300	8
HSP90AA1	1,668	35	Heat shock 90-kD protein 1, α
	151	6
HSPA8	1,338	39	Heat shock 70-kD protein 8 isoform 1
HSPA6	327	8	Heat shock 70-kD protein B
AHSA1	81	3	AHA-1 activator of heat shock 90-kD protein ATPase homologue 1
	27	9
DNAJA1	212	26	Hsp40 homologue, subfamily A, member 1
PSMD2	242	14	Proteasome 26S non-ATPase subunit 2
PSMD1	105	2	Proteasome 26S non-ATPase subunit 1
PSMD6	105	5	Proteasome 26S non-ATPase subunit 6
	46	6
PSMC2	75	3	Proteasome 26S ATPase subunit 2

HUGO name	Mascot score	Coverage	Description
		%
HSP90AB1	2,178	44	Heat shock 90-kD protein 1, β
	300	8
HSP90AA1	1,668	35	Heat shock 90-kD protein 1, α
	151	6
HSPA8	1,338	39	Heat shock 70-kD protein 8 isoform 1
HSPA6	327	8	Heat shock 70-kD protein B
AHSA1	81	3	AHA-1 activator of heat shock 90-kD protein ATPase homologue 1
	27	9
DNAJA1	212	26	Hsp40 homologue, subfamily A, member 1
PSMD2	242	14	Proteasome 26S non-ATPase subunit 2
PSMD1	105	2	Proteasome 26S non-ATPase subunit 1
PSMD6	105	5	Proteasome 26S non-ATPase subunit 6
	46	6
PSMC2	75	3	Proteasome 26S ATPase subunit 2

Proteins with two rows of data were identified in two independent experiments. The gel from one of the experiments is shown in Fig. 1 A. A threshold Mascot score of 50 was used as cut-off, indicating a 95% confidence of being a true identification. For AHSA1, in the second experiment, the mass spectrometry profile was manually examined to confirm the reliability of the observation.

[ViewLarge]

Sharing Unavailable