Table 1. Iron content of L. amazonensis... | Rockefeller University Press

Table 1.

Iron content of L. amazonensis cultured in iron-poor medium

Days of culture in iron-depleted medium	WT	Δlit1
	nmol/mg protein	nmol/mg protein
0	8.8 ± 0.7	8.7 ± 0.4
1	6.7 ± 0.6 (P = 0.0424)^a	5.5 ± 0.9 (P = 0.0158)^a
2	9.3 ± 1.9 (P = 0.1978)	5.8 ± 1.2 (P = 0.0798)
3	10.88 ± 1.7 (P = 0.0369)^a	5.9 ± 1.1 (P = 0.0451)^a

Days of culture in iron-depleted medium	WT	Δlit1
	nmol/mg protein	nmol/mg protein
0	8.8 ± 0.7	8.7 ± 0.4
1	6.7 ± 0.6 (P = 0.0424)^a	5.5 ± 0.9 (P = 0.0158)^a
2	9.3 ± 1.9 (P = 0.1978)	5.8 ± 1.2 (P = 0.0798)
3	10.88 ± 1.7 (P = 0.0369)^a	5.9 ± 1.1 (P = 0.0451)^a

Determination of total cellular iron content of L. amazonensis WT and Δlit1 parasites was performed using ferrozine-based quantitation (Riemer et al., 2004). The data represent the mean and SD of five independent experiments.

Indicates significant differences (P ≤ 0.05) from day 0 values (two-tailed Student’s t test).

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