Table 3. B cell populations in RAG2 −/−... | Rockefeller University Press

Table 3.

B cell populations in RAG2^−/−cγc^−/− mice 10 wk after transplantation with BM from SPPL2a^+/+ or SPPL2a^−/− mice

Cell type			SPPL2a^+/+		SPPL2a^−/−		P-value
			Mean	SD	Mean	SD
BM (% of viable cells)
B cells	B220⁺		11.8	3.3	10.9	5.3	0.694
Pro– /pre–B cells		B220⁺ IgM⁻	6.2	0.6	7.7	2.5	0.136
Immature B cells		B220⁺ IgM⁺	6.0	1.9	5.1	1.8	0.362
Recirculating B cells		B220^high	2.6	1.3	0.3	0.1	<0.001***
Spleen (% of viable cells)
B cells	B220⁺		41.6	1.3	20.8	6.9	<0.001***
T1 transitional		B220⁺ CD21^low CD24^high	6.4	1.7	11.1	3.8	0.018*
T2 transitional		B220⁺ CD21^high CD24^high	2.9	0.3	0.2	0.1	<0.001***
Mature B cells		B220⁺ CD21^low CD24^low	24.2	3.6	3.2	2.0	<0.001***
T helper cells		CD3⁺ CD4⁺	10.0	2.4	14.9	3.5	0.038*
Cytotoxic T cells		CD3⁺ CD8⁺	2.5	0.5	6.0	2.7	0.004**
Spleen (x10⁶ splenocytes)
B cells		B220⁺	42.5	7.9	11.3	5.2	<0.001***
T1 transitional		B220⁺ CD21^low CD24^high	7.3	1.5	5.7	2.4	0.300
T2 transitional		B220⁺ CD21^high CD24^high	3.0	0.2	0.1	0.1	<0.001***
Mature B cells		B220⁺ CD21^low CD24^low	22.7	4.2	1.9	1.5	<0.001***
T helper cells		CD3⁺ CD4⁺	9.6	2.7	7.8	3.3	0.194
Cytotoxic T cells		CD3⁺ CD8⁺	2.4	0.5	2.8	1.3	0.671

Cell type			SPPL2a^+/+		SPPL2a^−/−		P-value
			Mean	SD	Mean	SD
BM (% of viable cells)
B cells	B220⁺		11.8	3.3	10.9	5.3	0.694
Pro– /pre–B cells		B220⁺ IgM⁻	6.2	0.6	7.7	2.5	0.136
Immature B cells		B220⁺ IgM⁺	6.0	1.9	5.1	1.8	0.362
Recirculating B cells		B220^high	2.6	1.3	0.3	0.1	<0.001***
Spleen (% of viable cells)
B cells	B220⁺		41.6	1.3	20.8	6.9	<0.001***
T1 transitional		B220⁺ CD21^low CD24^high	6.4	1.7	11.1	3.8	0.018*
T2 transitional		B220⁺ CD21^high CD24^high	2.9	0.3	0.2	0.1	<0.001***
Mature B cells		B220⁺ CD21^low CD24^low	24.2	3.6	3.2	2.0	<0.001***
T helper cells		CD3⁺ CD4⁺	10.0	2.4	14.9	3.5	0.038*
Cytotoxic T cells		CD3⁺ CD8⁺	2.5	0.5	6.0	2.7	0.004**
Spleen (x10⁶ splenocytes)
B cells		B220⁺	42.5	7.9	11.3	5.2	<0.001***
T1 transitional		B220⁺ CD21^low CD24^high	7.3	1.5	5.7	2.4	0.300
T2 transitional		B220⁺ CD21^high CD24^high	3.0	0.2	0.1	0.1	<0.001***
Mature B cells		B220⁺ CD21^low CD24^low	22.7	4.2	1.9	1.5	<0.001***
T helper cells		CD3⁺ CD4⁺	9.6	2.7	7.8	3.3	0.194
Cytotoxic T cells		CD3⁺ CD8⁺	2.4	0.5	2.8	1.3	0.671

BM from SPPL2a^+/+ (n = 8) or SPPL2a^−/− (n = 8) mice was transplanted into irradiated RAG2^−/−cγc^−/− mice. Cells from BM and spleen of host mice were analyzed 10 wk after the transplantation by flow cytometry. Cell populations are expressed as percentage of viable cells (PI⁻, derived from n = 8) or absolute cell numbers (derived from n = 5), respectively. Data are listed as mean ± SD. ***, P < 0.001; **, P < 0.01; *, P < 0.05, unpaired Student’s t test.

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