Table 2. B cell subsets in different... | Rockefeller University Press

Table 2.

B cell subsets in different lymphatic tissues, blood and peritoneal cells of wild-type and SPPL2a-deficient mice

Cell type		SPPL2a^+/+		SPPL2a^−/−		P-value
		Mean	SD	Mean	SD
BM (% of viable cells)
B cells	B220⁺	21.4	6.1	15.3	2.5	0.031*
Pro– /pre–B cells	B220⁺ IgM⁻	8.5	1.9	10.3	2	0.099
Immature B cells	B220⁺ IgM⁺	3.6	1.9	4.1	2.3	0.694
Recirculating B cells	B220^high	8.6	3.6	0.7	0.2	<0.001***
Spleen (% of viable cells)
B cells	B220⁺	45.6	6.6	23.9	13.1	<0.001***
T1 transitional	B220⁺ CD21^low CD24^high	7.5	0.7	8.2	4.9	0.785
T2 transitional	B220⁺ CD21^high CD24^high	11.2	3.1	0.5	0.6	<0.001***
Mature B cells	B220⁺ CD21^low CD24^low	23.9	7.1	7.0	4.4	0.007**
Spleen (×10⁶ splenocytes)
B cells	B220⁺	33.3	13.5	4.2	2.2	0.021*
T1 transitional	B220⁺ CD21^low CD24^high	5.2	2.3	1.8	0.6	0.056
T2 transitional	B220⁺ CD21^high CD24^high	7.9	2.9	0.1	0.1	0.013*
Mature B cells	B220⁺ CD21^low CD24^low	17.8	7.3	1.8	1.2	0.021*
Peritoneal cells (% of viable cells)
B cells	B220⁺ CD19⁺	49.2	10.8	11.4	5.0	<0.001***
B1	B220^neg/low CD19^high	34.2	13.8	6.7	4.7	<0.001***
B2	B220^high CD19^+/low	15.1	6.9	2.1	1.7	0.001**

Cell type		SPPL2a^+/+		SPPL2a^−/−		P-value
		Mean	SD	Mean	SD
BM (% of viable cells)
B cells	B220⁺	21.4	6.1	15.3	2.5	0.031*
Pro– /pre–B cells	B220⁺ IgM⁻	8.5	1.9	10.3	2	0.099
Immature B cells	B220⁺ IgM⁺	3.6	1.9	4.1	2.3	0.694
Recirculating B cells	B220^high	8.6	3.6	0.7	0.2	<0.001***
Spleen (% of viable cells)
B cells	B220⁺	45.6	6.6	23.9	13.1	<0.001***
T1 transitional	B220⁺ CD21^low CD24^high	7.5	0.7	8.2	4.9	0.785
T2 transitional	B220⁺ CD21^high CD24^high	11.2	3.1	0.5	0.6	<0.001***
Mature B cells	B220⁺ CD21^low CD24^low	23.9	7.1	7.0	4.4	0.007**
Spleen (×10⁶ splenocytes)
B cells	B220⁺	33.3	13.5	4.2	2.2	0.021*
T1 transitional	B220⁺ CD21^low CD24^high	5.2	2.3	1.8	0.6	0.056
T2 transitional	B220⁺ CD21^high CD24^high	7.9	2.9	0.1	0.1	0.013*
Mature B cells	B220⁺ CD21^low CD24^low	17.8	7.3	1.8	1.2	0.021*
Peritoneal cells (% of viable cells)
B cells	B220⁺ CD19⁺	49.2	10.8	11.4	5.0	<0.001***
B1	B220^neg/low CD19^high	34.2	13.8	6.7	4.7	<0.001***
B2	B220^high CD19^+/low	15.1	6.9	2.1	1.7	0.001**

Cells of each genotype were analyzed by flow cytometry to determine the indicated cell populations as percentage of viable cells (PI⁻) or absolute cell numbers, respectively. BM, n = 7; spleen, n = 9; blood, n = 6; LNs n = 5; peritoneal cells, n = 12. Results are shown as mean ± SD. ***, P < 0.01; **, P < 0.01; *, P < 0.05, unpaired Student’s t test.

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