Table 1. Immune cells in different... | Rockefeller University Press

Table 1.

Immune cells in different lymphatic tissues and peritoneal cells of wild-type and SPPL2a-deficient mice

Cell type		SPPL2a^+/+		SPPL2a^−/−		P-value
		Mean	SD	Mean	SD
BM
B cells	B220⁺	21.4	6.1	15.3	2.5	0.031*
T cells	CD3⁺	1.8	1.2	1.1	1	0.066
Mature granulocytes	CD11b⁺ Gr1^high	63.4	9.6	70.5	1.4	0.137
Immature granulocytes	CD11b⁻ Gr1⁺	8.1	2.6	7.8	3.2	0.623
DCs	CD11c⁺	2.9	2.5	2.8	2	0.714
NK cells	CD49b⁺	2.6	0.6	2.1	0.7	0.078
Spleen
B cells	B220⁺	45.6	6.6	23.9	13.1	<0.001***
Follicular B cells	B220⁺ CD21⁺ CD23⁺	29.5	8.5	8.7	4.1	0.023*
Marginal zone	B220⁺ CD21⁺ CD23^−/low	4.6	1.6	1.3	0.2	0.026*
T cells	CD3⁺	32.7	4.4	38.3	3.6	0.01*
LNs
B cells	B220⁺	54.4	5	18	12.3	<0.001***
T cells	CD3⁺	35.7	4.2	59.5	11.4	0.018*
Macrophages	F4/80⁺	4.8	3.6	3.6	1.3	0.12
DCs	CD11c⁺	0.5	0.2	0.7	0.7	0.872
Blood
B cells	B220⁺	24.6	4.2	7.6	1.9	<0.001***
Peritoneal cells
B cells	B220⁺	49.2	10.8	11.4	5	<0.001***
Mast cells	Ckit^high ST2^high	1.8	0.8	3.8	2.2	0.061
Thymus
Double-positive T cells	CD4⁺ CD8⁺	78.8	4.3	81.5	5.7	0.289
CD4⁺ T cells	CD4⁺ CD8⁻	13.7	2.8	11.5	3.6	0.193
CD8⁺ T cells	CD4⁻ CD8⁺	3.4	1.2	3.1	1.4	0.582
Double-negative T cells	CD4⁻ CD8⁻	4.2	1.2	3.8	1.4	0.54

Cell type		SPPL2a^+/+		SPPL2a^−/−		P-value
		Mean	SD	Mean	SD
BM
B cells	B220⁺	21.4	6.1	15.3	2.5	0.031*
T cells	CD3⁺	1.8	1.2	1.1	1	0.066
Mature granulocytes	CD11b⁺ Gr1^high	63.4	9.6	70.5	1.4	0.137
Immature granulocytes	CD11b⁻ Gr1⁺	8.1	2.6	7.8	3.2	0.623
DCs	CD11c⁺	2.9	2.5	2.8	2	0.714
NK cells	CD49b⁺	2.6	0.6	2.1	0.7	0.078
Spleen
B cells	B220⁺	45.6	6.6	23.9	13.1	<0.001***
Follicular B cells	B220⁺ CD21⁺ CD23⁺	29.5	8.5	8.7	4.1	0.023*
Marginal zone	B220⁺ CD21⁺ CD23^−/low	4.6	1.6	1.3	0.2	0.026*
T cells	CD3⁺	32.7	4.4	38.3	3.6	0.01*
LNs
B cells	B220⁺	54.4	5	18	12.3	<0.001***
T cells	CD3⁺	35.7	4.2	59.5	11.4	0.018*
Macrophages	F4/80⁺	4.8	3.6	3.6	1.3	0.12
DCs	CD11c⁺	0.5	0.2	0.7	0.7	0.872
Blood
B cells	B220⁺	24.6	4.2	7.6	1.9	<0.001***
Peritoneal cells
B cells	B220⁺	49.2	10.8	11.4	5	<0.001***
Mast cells	Ckit^high ST2^high	1.8	0.8	3.8	2.2	0.061
Thymus
Double-positive T cells	CD4⁺ CD8⁺	78.8	4.3	81.5	5.7	0.289
CD4⁺ T cells	CD4⁺ CD8⁻	13.7	2.8	11.5	3.6	0.193
CD8⁺ T cells	CD4⁻ CD8⁺	3.4	1.2	3.1	1.4	0.582
Double-negative T cells	CD4⁻ CD8⁻	4.2	1.2	3.8	1.4	0.54

Cells from six mice of each genotype were analyzed by flow cytometry to determine the proportions of the indicated cell populations, which are shown as the percentage of viable cells (PI⁻). Results are listed as mean ± SD. ***, P < 0.001; *, P < 0.05, unpaired Student’s t test.

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