Table 2.

Antiviral activity of bNAbs on cell to cell transmission of two T/F HIV-1 strains

bNAbs IC50 
 WITO SUMA 
NIH45-46 3.1 13.9a 
3BNC60 3.4 3.3 
VRC01 14.3a 
8ANC195 3.7a 
10-1074 1.8 1.9 
PG16 0.05 0.3 
10E8 9.4a >15 
3BC176 
bNAbs IC50 
 WITO SUMA 
NIH45-46 3.1 13.9a 
3BNC60 3.4 3.3 
VRC01 14.3a 
8ANC195 3.7a 
10-1074 1.8 1.9 
PG16 0.05 0.3 
10E8 9.4a >15 
3BC176 

Primary HIV-1 CD4+ T cells were infected with two T/F HIV-1 strains (WITO or SUMA). Infected cells were preincubated for 1 h with different doses of bNAbs before co-culture with autologous target cells stained with FarRed dye. After 48 h, the fraction of productively infected (Gag+) target cells was measured by flow cytometry. The median inhibitory concentrations (IC50) were calculated with cells from three to four independent donors. Bold indicates IC50 < 0.5 µg/ml; italics indicate IC50 = 0.5–2 µg/ml; single underline indicates IC50 = 2–10 µg/ml; bold and underline indicate IC50 = 10–15 µg/ml. X, no neutralization (<25% inhibition at 15 µg/ml).

a

Partial neutralization (<90% inhibition at 15 µg/ml).

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