Analysis of Gata2 conditional knockout E14 FL
| E14 FL cell subset/cell type | Gata2f/+ or Gata2f/f | Vec-Cre:Gata2f/+ | Vec-Cre:Gata2f/f |
| % | % | % | |
| Differentiated cells | |||
| T cells | 5.1 ± 1.6 | 4.5 ± 1.5 | 3.3 ± 1.1 |
| B cells | 6.5 ± 1.4 | 7.5 ± 2.5 | 5.9 ± 1.5 |
| Erythroblasts | 61.7 ± 3.0 | 62.6 ± 1.3 | 61.9 ± 3.1 |
| Erythrocytes | 28.7 ± 3.1 | 30.3 ± 1.4 | 33.2 ± 3.3 |
| LSK-SLAM | 0.0150 ± 0.0021 | 0.0080 ± 0.0011 (P < 0.05) | 0.0066 ± 0.0017 (P < 0.01) |
| Total | |||
| G0 | 0.6 ± 0.2 | 0.6 ± 0.2 | 0.6 ± 0.3 |
| G1 | 44.3 ± 1.8 | 50.8 ± 2.7 | 44.8 ± 2.0 |
| S/G2/M | 54.5 ± 3.2 | 48.1 ± 2.6 | 54.1 ± 2.1 |
| Lin−cKithigh | |||
| G0 | 0.1 ± 0.03 | 0.2 ± 0.1 | 0.2 ± 0.05 |
| G1 | 31.5 ± 1.12 | 36.4 ± 3.9 | 35.5 ± 2.4 |
| S/G2/M | 67.9 ± 1.10 | 63.1 ± 3.8 | 63.9 ± 2.3 |
| Annexin+ | 13.8 ± 5.2 | 19.1 ± 5.1 | 45.2 ± 16.0 (P < 0.05) |
| E14 FL cell subset/cell type | Gata2f/+ or Gata2f/f | Vec-Cre:Gata2f/+ | Vec-Cre:Gata2f/f |
| % | % | % | |
| Differentiated cells | |||
| T cells | 5.1 ± 1.6 | 4.5 ± 1.5 | 3.3 ± 1.1 |
| B cells | 6.5 ± 1.4 | 7.5 ± 2.5 | 5.9 ± 1.5 |
| Erythroblasts | 61.7 ± 3.0 | 62.6 ± 1.3 | 61.9 ± 3.1 |
| Erythrocytes | 28.7 ± 3.1 | 30.3 ± 1.4 | 33.2 ± 3.3 |
| LSK-SLAM | 0.0150 ± 0.0021 | 0.0080 ± 0.0011 (P < 0.05) | 0.0066 ± 0.0017 (P < 0.01) |
| Total | |||
| G0 | 0.6 ± 0.2 | 0.6 ± 0.2 | 0.6 ± 0.3 |
| G1 | 44.3 ± 1.8 | 50.8 ± 2.7 | 44.8 ± 2.0 |
| S/G2/M | 54.5 ± 3.2 | 48.1 ± 2.6 | 54.1 ± 2.1 |
| Lin−cKithigh | |||
| G0 | 0.1 ± 0.03 | 0.2 ± 0.1 | 0.2 ± 0.05 |
| G1 | 31.5 ± 1.12 | 36.4 ± 3.9 | 35.5 ± 2.4 |
| S/G2/M | 67.9 ± 1.10 | 63.1 ± 3.8 | 63.9 ± 2.3 |
| Annexin+ | 13.8 ± 5.2 | 19.1 ± 5.1 | 45.2 ± 16.0 (P < 0.05) |
Values for differentiated cells and LSK-SLAM are percentage of viable cells. Values for Total and Lin2cKithigh are percentage of single cells. Values for Annexin+ are percentage of viable cells including pre-apoptotic. For the analysis of differentiated cells, n = 2 and number of embryos analyzed = 7, 4, and 8, respectively by genotype; LSK-SLAM, n = 5 and number of embryos analyzed = 12, 10, and 13, respectively; total and Lin−cKithigh, n = 2 and number of embryos analyzed = 7, 3, and 6; and LSK-SLAM Annexin+, n = 3 and number of embryos analyzed = 8, 10, and 9. Significance was determined by Student’s t test. ND = not done.