Table 1. Summary of mutation in... | Rockefeller University Press

Table 1.

Summary of mutation in transduced adoptive SW_HEL cells.

					Number of mutations
		Number of			G		C
Transduced cells	Transduced protein	Hosts	Sequences	Mutations	Tv	Ts	Tv	Ts	A	T
msh2^wt/wt SW_HEL	GFP	8	398	619	59	114	26	86	260	74
	ugi-GFP	4	208	471	6	147	6	161	113	38
	GFP-rag	4	173	231	26	42	17	37	97	12
	ugi-GFP-rag	5	218	332	3	103	2	87	112	25
	GFP-cdt	2	95	181	21	34	8	27	66	25
	ugi-GFP-cdt	2	65	114	2	28	0	46	27	11
	GFP-cyc	4	175	236	20	45	9	31	109	22
	ugi-GFP-cyc	4	243	307	20	73	6	72	102	34
	ugi-GFP-cyc^Dmut	4	141	269	3	90	1	81	68	26
msh2^ko/ko SW_HEL	GFP	3	152	141	18	38	4	74	6	1
	ugi-GFP	2	101	344	0	115	1	225	0	3
	ugi-GFP-rag	2	72	143	2	44	0	96	0	1
	ugi-GFP-cyc	3	90	91	5	35	0	47	4	0

					Number of mutations
		Number of			G		C
Transduced cells	Transduced protein	Hosts	Sequences	Mutations	Tv	Ts	Tv	Ts	A	T
msh2^wt/wt SW_HEL	GFP	8	398	619	59	114	26	86	260	74
	ugi-GFP	4	208	471	6	147	6	161	113	38
	GFP-rag	4	173	231	26	42	17	37	97	12
	ugi-GFP-rag	5	218	332	3	103	2	87	112	25
	GFP-cdt	2	95	181	21	34	8	27	66	25
	ugi-GFP-cdt	2	65	114	2	28	0	46	27	11
	GFP-cyc	4	175	236	20	45	9	31	109	22
	ugi-GFP-cyc	4	243	307	20	73	6	72	102	34
	ugi-GFP-cyc^Dmut	4	141	269	3	90	1	81	68	26
msh2^ko/ko SW_HEL	GFP	3	152	141	18	38	4	74	6	1
	ugi-GFP	2	101	344	0	115	1	225	0	3
	ugi-GFP-rag	2	72	143	2	44	0	96	0	1
	ugi-GFP-cyc	3	90	91	5	35	0	47	4	0

Table lists the number of host mice that received SW_HEL or msh2^ko/koSW_HEL B cells transduced to express the indicated protein, the total number of SW_HEL VDJ_H sequences recovered from those hosts, and the sum total of point mutations detected. These mutations are divided into tranversion (“Tv”) or transition (“Ts”) mutations at G or C, and mutations at A or T, referring to the nontemplate strand. The 523-bp sequence window examined is illustrated in Fig. 3 A.

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