Table 2. Stereotyped BCR in human PB... | Rockefeller University Press

Table 2.

Stereotyped BCR in human PB conventional, CD5⁺, and CD43⁺ B cells

Donorsanalyzed	Sample	V genes obtained	V genes included in analysis^a	Unique sequences^b	Stereotyped receptors^b	Fisher’s exact test^c
4^d	conventional	143	125	107	1
4^d	CD5⁺	460	419	160	12	P < 0.018
2^e	conventional	196	169	141	6
2^e	CD5⁺	180	158	145	18	P < 0.018
2^e	CD43⁺	115	72	12	0	P < 1

Donorsanalyzed	Sample	V genes obtained	V genes included in analysisa	Unique sequencesb	Stereotyped receptorsb	Fisher’s exact testc
4d	conventional	143	125	107	1
4d	CD5⁺	460	419	160	12	P < 0.018
2e	conventional	196	169	141	6
2e	CD5⁺	180	158	145	18	P < 0.018
2e	CD43⁺	115	72	12	0	P < 1

Only full-length V gene rearrangements and correct V-gene, i.e., VH1-69, VH3-21, VH3-23, VH3-48, and VH4-34 in stereotyped BCR-specific PCR approach

Identical sequences counted as one. When all sequences obtained are counted individually, the statistical significance is below P < 0.001. Stereotypy was determined based on a 60% homology of the CDR3.

P-values calculated by Fisher’s exact test versus unique conventional B cell sequences.

Total numbers of V_H1 and V_H3 gene analysis of four independent healthy blood donors are given, for details see Table S3.

Total numbers of V genes amplified by PCR for V_H gene segments frequently used by stereotyped BCR of two independent healthy blood donors are given, for details see Table S7.

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