Table 1. VH1 and VH3 gene analysis of... | Rockefeller University Press

Table 1.

VH1 and VH3 gene analysis of human PB CD5⁺ and conventional B cell subsets

Subset^a	Sequences	% mutated (range)	Average mutation frequency^b	Sequences assigned to clones	Number of clones
CD5⁺CD27⁻	148	5 (0–14)	0.04	115	31
CD5⁺IgM⁺CD27⁺	145	87 (76–95)	2.7	74	20
CD5⁺IgG/IgA⁺	126	82 (72–100)	2.17	116	15
CD5⁻ unmutated ^c	73	0	0	0	0
CD5⁻ mutated ^c	52	100	2.63	0	0

Subset^a	Sequences	% mutated (range)	Average mutation frequency^b	Sequences assigned to clones	Number of clones
CD5⁺CD27⁻	148	5 (0–14)	0.04	115	31
CD5⁺IgM⁺CD27⁺	145	87 (76–95)	2.7	74	20
CD5⁺IgG/IgA⁺	126	82 (72–100)	2.17	116	15
CD5⁻ unmutated ^c	73	0	0	0	0
CD5⁻ mutated ^c	52	100	2.63	0	0

For CD5⁺ and conventional subsets total numbers of four and two healthy donors are given, respectively.

For calculation of the average mutation frequency, identical sequences were counted once, as they might derive from one cell. If all sequences were considered, very similar values were obtained (Table S3). In case of intraclonal diversity, each unique sequence was regarded as derived from an independent cell and counted once. Both in-frame and out-of-frame rearrangements were considered.

CD5⁻, conventional B cells were isolated and analyzed as CD19⁺ B cells and, afterward, separated into mutated and unmutated sequences.

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