Sizes of Peyer’s patch, MLN, IEL, and LPL subpopulations
| WT | EE | |
| PP | ||
| CD4+ | 11.25 ± 1.3 | 12 ± 1.4 |
| CD8+ | 3.75 ± 0.5 | 3.4 ± 0.3 |
| B220+ | 86 ± 5.1 | 87 ± 7.2 |
| CD11c+ | 0.5 ± 0.1 | 0.3 ± 0.2 |
| MLN | ||
| CD4+ | 31 ± 2.5 | 30 ± 1.7 |
| CD8+ | 21 ± 1.8 | 20 ± 1.2 |
| B220+ | 26 ± 3.5 | 28 ± 2.1 |
| CD11c+ | 4 ± 0.5 | 3 ± 0.7 |
| IELs | ||
| Percentage within CD3+ cells: | ||
| TCRαβ+ | 45.2 ± 3.4 | 45.1 ± 2.3 |
| TCRγδ+ | 54.8 ± 4.5 | 54.9 ± 4.3 |
| Percentage within TCRαβ+ cells: | ||
| CD8αα+ CD8αβ- | 41.2 ± 2.3 | 37 ± 3.4 |
| CD8αβ+ | 30.1 ± 1.5 | 30.2 ± 2.5 |
| CD4+ | 24 ± 1.3 | 22 ± 1.5 |
| Percentage within TCRγδ+ cells: | ||
| CD8αα+ CD8αβ- | 83 ± 3.2 | 82 ± 2.4 |
| CD8αβ+ | 4.1 ± 1.1 | 4.2 ± 0.9 |
| LPLs | ||
| Percentage within TCRαβ+ cells: | ||
| CD8αβ+ | 15.3 ± 1.4 | 12.7 ± 2.8 |
| CD4+ | 78.2 ± 3.4 | 78.4 ± 2.9 |
| CD4+Foxp3+ | 17.2 ± 2.3 | 18.1 ± 3.2 |
| WT | EE | |
| PP | ||
| CD4+ | 11.25 ± 1.3 | 12 ± 1.4 |
| CD8+ | 3.75 ± 0.5 | 3.4 ± 0.3 |
| B220+ | 86 ± 5.1 | 87 ± 7.2 |
| CD11c+ | 0.5 ± 0.1 | 0.3 ± 0.2 |
| MLN | ||
| CD4+ | 31 ± 2.5 | 30 ± 1.7 |
| CD8+ | 21 ± 1.8 | 20 ± 1.2 |
| B220+ | 26 ± 3.5 | 28 ± 2.1 |
| CD11c+ | 4 ± 0.5 | 3 ± 0.7 |
| IELs | ||
| Percentage within CD3+ cells: | ||
| TCRαβ+ | 45.2 ± 3.4 | 45.1 ± 2.3 |
| TCRγδ+ | 54.8 ± 4.5 | 54.9 ± 4.3 |
| Percentage within TCRαβ+ cells: | ||
| CD8αα+ CD8αβ- | 41.2 ± 2.3 | 37 ± 3.4 |
| CD8αβ+ | 30.1 ± 1.5 | 30.2 ± 2.5 |
| CD4+ | 24 ± 1.3 | 22 ± 1.5 |
| Percentage within TCRγδ+ cells: | ||
| CD8αα+ CD8αβ- | 83 ± 3.2 | 82 ± 2.4 |
| CD8αβ+ | 4.1 ± 1.1 | 4.2 ± 0.9 |
| LPLs | ||
| Percentage within TCRαβ+ cells: | ||
| CD8αβ+ | 15.3 ± 1.4 | 12.7 ± 2.8 |
| CD4+ | 78.2 ± 3.4 | 78.4 ± 2.9 |
| CD4+Foxp3+ | 17.2 ± 2.3 | 18.1 ± 3.2 |
Peyer’s patches (PP), MLN, IEL, and LPL subpopulations. Isolated cells from MLN, small intestinal PP, LPLs and IELs of WT or IKKβ(EE)IEC (n = 4) were stained with labeled antibodies and analyzed by FACS.