Loss of E2A in SS tumor cells
| Patient no. | Loss of E2A | Purity of enriched tumor cells | |
| Array CGH analysis | FISH analysis (number of cells with deletion/number of analyzed cells) | ||
| % | |||
| 1 | yesc | yes (149/200)e | 96.7 |
| 2 | yesc | n.d | – |
| 3 | yesc | yes (198/200)e | 99.1 |
| 4a | yesc | yes (167/200)e | 93.2 |
| 5 | yese | yes (90/200)e | 97.9 |
| 6 | yesd | n.d. | – |
| 7a | yesc | no (14/200)e | 99.2f |
| 8 | yese | n.d. | – |
| 9 | noc | no (3/200)e | 97.4 |
| 10 | noc | no (4/200)e | 96.9 |
| 11a | yese | no (12/200)d | n.d. |
| 12 | noc | n.d. | – |
| 13 | yesc | yes (126/200)e | 98.4f |
| 14 | noc | n.d. | – |
| 15a | nobc | yes (106/200)d | n.d. |
| 16a | noe | yes (loss 28/200, gain 46/200)e | 97.2 |
| 17 | yese | yes (50/200)e | 98.4 |
| 18 | yesc | n.d. | – |
| 19 | yese | n.d. | – |
| 20 | yese | n.d. | – |
| Se-Ax cell line | yes | yes (190/200) | |
| Patient no. | Loss of E2A | Purity of enriched tumor cells | |
| Array CGH analysis | FISH analysis (number of cells with deletion/number of analyzed cells) | ||
| % | |||
| 1 | yesc | yes (149/200)e | 96.7 |
| 2 | yesc | n.d | – |
| 3 | yesc | yes (198/200)e | 99.1 |
| 4a | yesc | yes (167/200)e | 93.2 |
| 5 | yese | yes (90/200)e | 97.9 |
| 6 | yesd | n.d. | – |
| 7a | yesc | no (14/200)e | 99.2f |
| 8 | yese | n.d. | – |
| 9 | noc | no (3/200)e | 97.4 |
| 10 | noc | no (4/200)e | 96.9 |
| 11a | yese | no (12/200)d | n.d. |
| 12 | noc | n.d. | – |
| 13 | yesc | yes (126/200)e | 98.4f |
| 14 | noc | n.d. | – |
| 15a | nobc | yes (106/200)d | n.d. |
| 16a | noe | yes (loss 28/200, gain 46/200)e | 97.2 |
| 17 | yese | yes (50/200)e | 98.4 |
| 18 | yesc | n.d. | – |
| 19 | yese | n.d. | – |
| 20 | yese | n.d. | – |
| Se-Ax cell line | yes | yes (190/200) | |
n.d.: not determined
CGH and FISH analyses were performed with samples from different time points (see Table S1).
hypotriploid cells, gain of chromosome 19 except 19p13.2-13.3.
Analysis of PBMC cells.
Analysis of CD4-sorted cells.
Analysis of Vβ-sorted cells.
Purity was determined with CD4 antibody only.