Depletion of NK cells and macrophages by anti-CD122 Ab or CLO treatment
| Treatment | NK | MAC | ||
| BM | SP | BM | SP | |
| % | % | % | % | |
| NS mice | ||||
| PBS | 2.7 ± 0.1 (3) | 19.7 ± 1.6 (3) | 11.0 ± 2.9 (3) | 46.3 ± 1.0 (3) |
| CD122 | 0.9 ± 0.1 (3)c | 10.3 ± 1.0 (3)c | 11.0 ± 1.4 (3) | 54.5 ± 1.4 (3)c |
| CLO | 3.0 ± 0.2 (5) | 34.0 ± 2.1 (5)c | 1.7 ± 0.2 (4)b | 2.1 ± 0.2 (4)c |
| NS-Idd13 mice | ||||
| PBS | 2.7 ± 0.7 (3) | 22.9 ± 1.3 (3) | 4.8 ± 1.2 (3) | 37.3 ± 1.5 (3) |
| CD122 | 0.8 ± 0.2 (3)a | 7.8 ± 1.1 (3)c | 6.6 ± 1.5 (3) | 48.1 ± 1.4 (3)c |
| CLO | 3.0 ± 0.4 (5) | 25.7 ± 3.2 (5) | 1.6 ± 0.2 (5)a | 2.6 ± 0.3 (5)c |
| Treatment | NK | MAC | ||
| BM | SP | BM | SP | |
| % | % | % | % | |
| NS mice | ||||
| PBS | 2.7 ± 0.1 (3) | 19.7 ± 1.6 (3) | 11.0 ± 2.9 (3) | 46.3 ± 1.0 (3) |
| CD122 | 0.9 ± 0.1 (3)c | 10.3 ± 1.0 (3)c | 11.0 ± 1.4 (3) | 54.5 ± 1.4 (3)c |
| CLO | 3.0 ± 0.2 (5) | 34.0 ± 2.1 (5)c | 1.7 ± 0.2 (4)b | 2.1 ± 0.2 (4)c |
| NS-Idd13 mice | ||||
| PBS | 2.7 ± 0.7 (3) | 22.9 ± 1.3 (3) | 4.8 ± 1.2 (3) | 37.3 ± 1.5 (3) |
| CD122 | 0.8 ± 0.2 (3)a | 7.8 ± 1.1 (3)c | 6.6 ± 1.5 (3) | 48.1 ± 1.4 (3)c |
| CLO | 3.0 ± 0.4 (5) | 25.7 ± 3.2 (5) | 1.6 ± 0.2 (5)a | 2.6 ± 0.3 (5)c |
Percentage of NK cells (F4/80−CD49b+) and macrophages (MAC; F4/80+CD49b−) harvested from the BM and SP of NS and NS-Idd13 mice after treatment with control liposomes containing PBS, anti-CD122 Ab (CD122), or CLO liposomes. Data are expressed as mean ± SEM (n mice). CD122 and CLO treatment resulted in depletion of NK cells and macrophages, respectively, with concomitant enrichment of the opposite cell population in some cases.
*, P < 0.05 versus PBS (ANOVA).
**, P < 0.01 versus PBS (ANOVA).
***, P < 0.001 versus PBS (ANOVA).