Vβ6-Jβ2.1 joint of HEL12–27-specific CD4 T cells
| Sequences (aa) | L | nCh | WT | Tssp−/− | |||||||
| 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | 5 | |||
| SRTDYAEQ | 8 | −1 | 2 | 4 | 1 | ||||||
| SIRTGGFEQ | 9 | 0 | 1 | ||||||||
| SRQGGDTQY | 9 | 0 | 1 | ||||||||
| SIWGGNYAEQ | 10 | −1 | 1 | ||||||||
| SSRDRNYAEQ | 10 | 0 | 1 | ||||||||
| SIRDRNYAEQ | 10 | 0 | 1 | ||||||||
| FPRIPGKNYAEQ | 12 | 1 | 1 | ||||||||
| SISNYAEQ | 8 | −1 | 1 | 1 | 8 | 8 | 1 | 1 | 1 | 1 | |
| SIWDRYAEQ | 9 | −1 | 2 | 2 | 1 | ||||||
| STGGNYAEQ | 9 | −1 | 2 | 3 | |||||||
| SILGGNYAEQ | 10 | −1 | 2 | 4 | 3 | 6 | 4 | 5 | 2 | 2 | |
| RGQGNNYAEQ | 10 | 0 | 2 | 1 | 1 | 1 | |||||
| TLTPGAEQ | 8 | −1 | 1 | ||||||||
| RQQNYAEQ | 8 | 0 | 1 | ||||||||
| SIWDGYAEQ | 9 | −2 | 1 | ||||||||
| PIQENYAEQ | 9 | −2 | 2 | ||||||||
| SILWGPYEQY | 10 | −1 | 1 | ||||||||
| RGQGNRCAEQ | 10 | 1 | 1 | ||||||||
| SIVWGGIDAEQ | 11 | −2 | 1 | ||||||||
| SPDWGRNYAEQ | 11 | −1 | 2 | 4 | |||||||
| Sequences (aa) | L | nCh | WT | Tssp−/− | |||||||
| 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | 5 | |||
| SRTDYAEQ | 8 | −1 | 2 | 4 | 1 | ||||||
| SIRTGGFEQ | 9 | 0 | 1 | ||||||||
| SRQGGDTQY | 9 | 0 | 1 | ||||||||
| SIWGGNYAEQ | 10 | −1 | 1 | ||||||||
| SSRDRNYAEQ | 10 | 0 | 1 | ||||||||
| SIRDRNYAEQ | 10 | 0 | 1 | ||||||||
| FPRIPGKNYAEQ | 12 | 1 | 1 | ||||||||
| SISNYAEQ | 8 | −1 | 1 | 1 | 8 | 8 | 1 | 1 | 1 | 1 | |
| SIWDRYAEQ | 9 | −1 | 2 | 2 | 1 | ||||||
| STGGNYAEQ | 9 | −1 | 2 | 3 | |||||||
| SILGGNYAEQ | 10 | −1 | 2 | 4 | 3 | 6 | 4 | 5 | 2 | 2 | |
| RGQGNNYAEQ | 10 | 0 | 2 | 1 | 1 | 1 | |||||
| TLTPGAEQ | 8 | −1 | 1 | ||||||||
| RQQNYAEQ | 8 | 0 | 1 | ||||||||
| SIWDGYAEQ | 9 | −2 | 1 | ||||||||
| PIQENYAEQ | 9 | −2 | 2 | ||||||||
| SILWGPYEQY | 10 | −1 | 1 | ||||||||
| RGQGNRCAEQ | 10 | 1 | 1 | ||||||||
| SIVWGGIDAEQ | 11 | −2 | 1 | ||||||||
| SPDWGRNYAEQ | 11 | −1 | 2 | 4 | |||||||
Compilation of 18 distinct CDR3 sequences derived from 92 clones of Vβ6-Jβ2.1 joints from purified HEL-responsive CD4 T cells from four WT (1–4) and five Tssp−/− (1–5) HEL-immunized mice. For each sequence, the CDR3 length (L) and CDR3 net charge (nCh) are indicated. Negatively and positively charged amino acids are shown underlined and in bold, respectively. The number of times each sequence occurred in individual mice is shown.