Table III.

Apparent binding affinity of observed, RUA, and intermediate antibodies in clonal lineage 558 to HIV-1 gp41

Antibody ID EC50 Apparent Kd 
 µg/ml nM 
H789-K584 0.7 4.7 
H684-K462 56.3 375.3 
H828-K616 90 0.6 
CL558_RUA NB NA 
CL558_1 NB NA 
CL558_2 26.7 0.2 
CL558_5 22.8 152 
CL558_6 9.5 65.3 
CL558_7 7.8 52 
CL558_9 25.7 171.3 
CL558_13 18.2 121.3 
CL558_14 21.9 146 
CL558_15 5.3 35.3 
CL558_16 2.1 14 
Antibody ID EC50 Apparent Kd 
 µg/ml nM 
H789-K584 0.7 4.7 
H684-K462 56.3 375.3 
H828-K616 90 0.6 
CL558_RUA NB NA 
CL558_1 NB NA 
CL558_2 26.7 0.2 
CL558_5 22.8 152 
CL558_6 9.5 65.3 
CL558_7 7.8 52 
CL558_9 25.7 171.3 
CL558_13 18.2 121.3 
CL558_14 21.9 146 
CL558_15 5.3 35.3 
CL558_16 2.1 14 

Binding of antibodies to HIV-1 gp41 was determined in Luminex assays at antibody concentrations ranging from 50 to 0.2 µg/ml. NB, No detectable binding; NA, not applicable.

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