Reduction of BAFF activity reduces the infiltration of leukocytes into the kidneys of lyn−/− mice
| Treatment | lyn−/− | lyn−/− + anti-BAFF antibody |
| CD45+ (×105) | 18.5 ± 4 | 9.8 ± 1.4* |
| CD45+CD19+ (×105) | 0.9 ± 0.2 | 0.15 ± 0.05** |
| CD45+Mac-1+ (×105) | 12.3 ± 4 | 5.1 ± 0.6* |
| CD45+TCRβ+ (×105) | 5.2 ± 0.8 | 4.5 ± 0.5 |
| CD45+TCRβ+CD44highCD62L− (%) | 77 ± 2 | 52 ± 5*** |
| Histological score | ||
| Glomerulonephritis | 2.2 ± 0.3 | 1.1 ± 0.21** |
| Interstitial nephritis | 2 ± 0.2 | 0.6 ± 0.1*** |
| Immunofluorescence staining intensity | ||
| IgM deposit score | +++ | +++ |
| IgG deposit score | ++ | +/− |
| C3 deposit score | +++ | +/− |
| Treatment | lyn−/− | lyn−/− + anti-BAFF antibody |
| CD45+ (×105) | 18.5 ± 4 | 9.8 ± 1.4* |
| CD45+CD19+ (×105) | 0.9 ± 0.2 | 0.15 ± 0.05** |
| CD45+Mac-1+ (×105) | 12.3 ± 4 | 5.1 ± 0.6* |
| CD45+TCRβ+ (×105) | 5.2 ± 0.8 | 4.5 ± 0.5 |
| CD45+TCRβ+CD44highCD62L− (%) | 77 ± 2 | 52 ± 5*** |
| Histological score | ||
| Glomerulonephritis | 2.2 ± 0.3 | 1.1 ± 0.21** |
| Interstitial nephritis | 2 ± 0.2 | 0.6 ± 0.1*** |
| Immunofluorescence staining intensity | ||
| IgM deposit score | +++ | +++ |
| IgG deposit score | ++ | +/− |
| C3 deposit score | +++ | +/− |
Young (2-mo-old) lyn−/− mice were treated with neutralizing anti-BAFF mAb or irrelevant isotype control mAb for 5 mo (long-term treatment), as described in Materials and methods. Mice were sacrificed, and kidneys were isolated, processed, and analyzed for infiltration of leukocytes by flow cytometry, as described in Materials and methods. The absolute number of total leukocytes (CD45+), B cells (CD19+), myeloid cells (Mac-1+), and T cells (TCRβ+), and the percentage of activated effector T cells (TCRβ+CD44highCD62L−) are reported as means ± SEM (n = 7–10). *, P < 0.05; **, P < 0.01; ***, P < 0.001. Histological analysis of renal disease as well as of Ig immune complex and C3 deposit immunofluorescent staining intensity was performed as described in Materials and methods. Data are representative of 12 mice for each genotype analyzed at end-point experiments.