Increased infiltration of leukocytes into the kidneys of lyn−/− mice and lyn−/−rag−/− chimeras
| Regular mice | Bone marrow chimeras | ||||||
| Genotype | WT | lyn−/− | HFL−/− | lyn−/− IFNγ−/− | WT | rag−/− | lyn−/−rag−/− |
| CD45+ (×105) | 7.5 ± 1.5 | 22 ± 3 | 9.5 ± 1*** | 10.7 ± 1*** | 8.2 ± 1.7 | 9.7 ± 1 | 15.5 ± 1** |
| CD45+CD19+ (×105) | 2 ± 0.4 | 0.9 ± 0.4 | 1.5 ± 0.5 | 1 ± 0.3 | 1.9 ± 0.2 | 1.2 ± 0.1 | 1 ± 0.1 |
| CD45+Mac-1+ (×105) | 3 ± 0.6 | 15 ± 2 | 4 ± 1*** | 5.6 ± 1*** | 3. ± 0.3 | 4 ± 0.3 | 9.2 ± 1** |
| CD45+TCRβ+ (×105) | 2.5 ± 0.5 | 6 ± 1 | 3.5 ± 0.6* | 4 ± 0.6* | 3.2 ± 0.6 | 4.2 ± 0.6 | 5.1 ± 0.7 |
| CD45+TCRβ+CD44highCD62L− (%) | 35 ± 4 | 80 ± 5 | 45 ± 5*** | 50 ± 3*** | 42 ± 3 | 51 ± 6 | 75 ± 5** |
| Histological score | |||||||
| Glomerulonephritis | 0 | 2.8 ± 0.1 | 1.7 ± 0.1** | 1.1 ± 0.2*** | 0 | 0.6 ± 0.1 | 1.6 ± 0.12* |
| Interstitial nephritis | 0 | 2.5 ± 0.2 | 1.1 ± 0.1*** | 0.5 ± 0.1*** | 0 | 0.2 ± 0.04 | 2.3 ± 0.2** |
| Immunofluorescence staining intensity | |||||||
| IgM deposit score | +/− | +++ | +++ | +++ | +/− | ++ | +++ |
| IgG deposit score | − | ++ | + | +/− | - | - | − |
| C3 deposit score | − | +++ | + | +/− | - | - | − |
| Regular mice | Bone marrow chimeras | ||||||
| Genotype | WT | lyn−/− | HFL−/− | lyn−/− IFNγ−/− | WT | rag−/− | lyn−/−rag−/− |
| CD45+ (×105) | 7.5 ± 1.5 | 22 ± 3 | 9.5 ± 1*** | 10.7 ± 1*** | 8.2 ± 1.7 | 9.7 ± 1 | 15.5 ± 1** |
| CD45+CD19+ (×105) | 2 ± 0.4 | 0.9 ± 0.4 | 1.5 ± 0.5 | 1 ± 0.3 | 1.9 ± 0.2 | 1.2 ± 0.1 | 1 ± 0.1 |
| CD45+Mac-1+ (×105) | 3 ± 0.6 | 15 ± 2 | 4 ± 1*** | 5.6 ± 1*** | 3. ± 0.3 | 4 ± 0.3 | 9.2 ± 1** |
| CD45+TCRβ+ (×105) | 2.5 ± 0.5 | 6 ± 1 | 3.5 ± 0.6* | 4 ± 0.6* | 3.2 ± 0.6 | 4.2 ± 0.6 | 5.1 ± 0.7 |
| CD45+TCRβ+CD44highCD62L− (%) | 35 ± 4 | 80 ± 5 | 45 ± 5*** | 50 ± 3*** | 42 ± 3 | 51 ± 6 | 75 ± 5** |
| Histological score | |||||||
| Glomerulonephritis | 0 | 2.8 ± 0.1 | 1.7 ± 0.1** | 1.1 ± 0.2*** | 0 | 0.6 ± 0.1 | 1.6 ± 0.12* |
| Interstitial nephritis | 0 | 2.5 ± 0.2 | 1.1 ± 0.1*** | 0.5 ± 0.1*** | 0 | 0.2 ± 0.04 | 2.3 ± 0.2** |
| Immunofluorescence staining intensity | |||||||
| IgM deposit score | +/− | +++ | +++ | +++ | +/− | ++ | +++ |
| IgG deposit score | − | ++ | + | +/− | - | - | − |
| C3 deposit score | − | +++ | + | +/− | - | - | − |
Kidneys were isolated from 8–12-mo-old mice (either regular animals or bone marrow chimeras) of the indicated genotypes, processed, and analyzed for infiltration of leukocytes by flow cytometry, as described in Materials and methods. The absolute number of total leukocytes (CD45+), B cells (CD19+), myeloid cells (Mac-1+), and T cells (TCRβ+), and the percentage of activated effector T cells (TCRβ+CD44highCD62L−) are reported as means ± SEM (n = 8–12). Statistical differences between HFL−/− or lyn−/−IFNγ−/− versus lyn−/− mice are reported. Similarly, statistical differences between rag−/−-WT versus lyn−/−rag−/−-WT chimeras are reported. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Histological analysis of renal disease as well as of Ig immune complex and C3 deposit immunofluorescent staining intensity was performed as described in Materials and methods. Data are representative of 10–20 mice for each genotype analyzed at end-point experiments.