Gating parameters for CNGA3 mutations
| CNGA3 | K1/2 cGMP (µM) | Hill coefficient | Po max cGMP | Imax cAMP/Imax cGMP |
| Wild type | 9.29 ± 0.43 (18) | 1.91 ± 0.04 (18) | 0.97 ± 0.01 (10) | 0.14 ± 0.01 (20) |
| 613X | 12.68 ± 0.99 (11)a | 1.55 ± 0.09 (11) | 0.93 ± 0.01 (4) | 0.12 ± 0.01 (16) |
| 635X | 8.20 ± 0.57 (5) | 2.00 ± 0.00 (5) | 0.92 ± 0.01 (4) | 0.18 ± 0.02 (5) |
| 655X | 8.83 ± 0.26 (3) | 1.73 ± 0.10 (3) | 0.94 ± 0.01 (4) | 0.15 ± 0.02 (4) |
| 675X | 9.22 ± 0.55 (4) | 1.88 ± 0.05 (4) | ND | 0.15 ± 0.02 (6) |
| C7K/R-A | 9.37 ± 0.29 (6) | 2.00 ± 0.00 (6) | 0.92 ± 0.01 (4) | 0.16 ± 0.01 (6) |
| RR-QQ | 7.45 ± 0.63 (3) | 2.00 ± 0.00 (3) | 0.94 ± 0.01 (3) | 0.15 ± 0.01 (13) |
| RR-EE | 8.29 ± 0.47 (4) | 1.95 ± 0.05 (4) | 0.93 ± 0.03 (4) | 0.17 ± 0.01 (10) |
| P99L, A105P | 5.30 ± 0.18 (7)a | 2.00 ± 0.00 (7) | 0.95 ± 0.01 (4) | 0.21 ± 0.01 (7)a |
| ΔN (2-156) | 9.16 ± 0.65 (9) | 1.77 ± 0.07 (9) | ND | 0.18 ± 0.01 (13)a |
| ΔN (2-156), 613X | 15.75 ± 2.80 (6)a | 1.66 ± 0.10 (6) | ND | 0.10 ± 0.02 (6) |
| ΔN (51-107), 613X | 11.14 ± 2.28 (4) | 1.90 ± 0.06 (4) | ND | 0.10 ± 0.01 (4) |
| N7R-A | 8.89 ± 1.15 (4) | 1.59 ± 0.18 (4) | ND | 0.11 ± 0.01 (4) |
| N7R-A, 613X | 16.36 ± 1.65 (6)a | 1.82 ± 0.04 (6) | ND | 0.10 ± 0.04 (4) |
| N5R-A, 613X | 16.74 ± 0.98 (8)a | 1.71 ± 0.05 (8) | ND | 0.10 ± 0.01 (8) |
| N2R-A, 613X | 16.10 ± 0.59 (4)a | 1.68 ± 0.03 (4) | ND | 0.11 ± 0.01 (4) |
| CNGA3 | K1/2 cGMP (µM) | Hill coefficient | Po max cGMP | Imax cAMP/Imax cGMP |
| Wild type | 9.29 ± 0.43 (18) | 1.91 ± 0.04 (18) | 0.97 ± 0.01 (10) | 0.14 ± 0.01 (20) |
| 613X | 12.68 ± 0.99 (11)a | 1.55 ± 0.09 (11) | 0.93 ± 0.01 (4) | 0.12 ± 0.01 (16) |
| 635X | 8.20 ± 0.57 (5) | 2.00 ± 0.00 (5) | 0.92 ± 0.01 (4) | 0.18 ± 0.02 (5) |
| 655X | 8.83 ± 0.26 (3) | 1.73 ± 0.10 (3) | 0.94 ± 0.01 (4) | 0.15 ± 0.02 (4) |
| 675X | 9.22 ± 0.55 (4) | 1.88 ± 0.05 (4) | ND | 0.15 ± 0.02 (6) |
| C7K/R-A | 9.37 ± 0.29 (6) | 2.00 ± 0.00 (6) | 0.92 ± 0.01 (4) | 0.16 ± 0.01 (6) |
| RR-QQ | 7.45 ± 0.63 (3) | 2.00 ± 0.00 (3) | 0.94 ± 0.01 (3) | 0.15 ± 0.01 (13) |
| RR-EE | 8.29 ± 0.47 (4) | 1.95 ± 0.05 (4) | 0.93 ± 0.03 (4) | 0.17 ± 0.01 (10) |
| P99L, A105P | 5.30 ± 0.18 (7)a | 2.00 ± 0.00 (7) | 0.95 ± 0.01 (4) | 0.21 ± 0.01 (7)a |
| ΔN (2-156) | 9.16 ± 0.65 (9) | 1.77 ± 0.07 (9) | ND | 0.18 ± 0.01 (13)a |
| ΔN (2-156), 613X | 15.75 ± 2.80 (6)a | 1.66 ± 0.10 (6) | ND | 0.10 ± 0.02 (6) |
| ΔN (51-107), 613X | 11.14 ± 2.28 (4) | 1.90 ± 0.06 (4) | ND | 0.10 ± 0.01 (4) |
| N7R-A | 8.89 ± 1.15 (4) | 1.59 ± 0.18 (4) | ND | 0.11 ± 0.01 (4) |
| N7R-A, 613X | 16.36 ± 1.65 (6)a | 1.82 ± 0.04 (6) | ND | 0.10 ± 0.04 (4) |
| N5R-A, 613X | 16.74 ± 0.98 (8)a | 1.71 ± 0.05 (8) | ND | 0.10 ± 0.01 (8) |
| N2R-A, 613X | 16.10 ± 0.59 (4)a | 1.68 ± 0.03 (4) | ND | 0.11 ± 0.01 (4) |
The numbers in parentheses after the gating parameters indicate the number of patches (n).
Significant difference compared to wild-type CNGA3 channels (P < 0.05).