Expression of SIY and HEL antigens on reisolated tumors
| Inoculation on side A | Inoculation on side B | Animal number | Tumor on side B | |||
| Days of growth | Volume | Antigen expression (% of tumor cells positive) | ||||
| SIY only | HEL only | |||||
| mm3 | % | % | ||||
| Experiment I | ||||||
| A-SIY/HEL | A-SIY + A-HEL | 1 | 21 | 2,600 | <1 | 93 |
| A-SIY/HEL | A-SIY + A-HEL | 2 | 21 | 1,950 | <1 | 96 |
| A-SIY/HEL | A-SIY + A-HEL | 3 | 21 | 4,900 | <1 | 89 |
| A-SIY/HEL | A-SIY + A-HEL | 4 | 21 | 2,600 | <1 | 95 |
| None | A-SIY + A-HEL | 1 | 18 | 1,300 | <1 | 94 |
| None | A-SIY + A-HEL | 2 | 18 | 1,200 | <1 | 95 |
| Experiment II | ||||||
| A-SIY/HEL | A-SIY | 1 | 26 | 520 | 80 | NA |
| A-SIY/HEL | A-SIY | 2 | 26 | 644 | 83 | NA |
| A-SIY/HEL | A-SIY | 3 | 29 | 520 | 82 | NA |
| Inoculation on side A | Inoculation on side B | Animal number | Tumor on side B | |||
| Days of growth | Volume | Antigen expression (% of tumor cells positive) | ||||
| SIY only | HEL only | |||||
| mm3 | % | % | ||||
| Experiment I | ||||||
| A-SIY/HEL | A-SIY + A-HEL | 1 | 21 | 2,600 | <1 | 93 |
| A-SIY/HEL | A-SIY + A-HEL | 2 | 21 | 1,950 | <1 | 96 |
| A-SIY/HEL | A-SIY + A-HEL | 3 | 21 | 4,900 | <1 | 89 |
| A-SIY/HEL | A-SIY + A-HEL | 4 | 21 | 2,600 | <1 | 95 |
| None | A-SIY + A-HEL | 1 | 18 | 1,300 | <1 | 94 |
| None | A-SIY + A-HEL | 2 | 18 | 1,200 | <1 | 95 |
| Experiment II | ||||||
| A-SIY/HEL | A-SIY | 1 | 26 | 520 | 80 | NA |
| A-SIY/HEL | A-SIY | 2 | 26 | 644 | 83 | NA |
| A-SIY/HEL | A-SIY | 3 | 29 | 520 | 82 | NA |
NA, not applicable. 5 × 105 A-SIY/HEL or A-SIY cancer cells or 2.5 × 105 A-SIY mixed with 2.5 × 105 A-HEL cancer cells were injected subcutaneously into opposite flanks of B6C3 mice as indicated. Tumor growth was monitored. Tumors on side B were readapted to culture and analyzed by flow cytometry for expression of the SIY and HEL antigens. Data are representative of at least two independent experiments.